Application of Group I Metal Adduction to the Separation of Steroids by Traveling Wave Ion Mobility Spectrometry

Alana L. Rister, Tiana L. Martin, Eric D. Dodds

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Steroids represent an interesting class of small biomolecules due to their use as biomarkers and their status as scheduled drugs. Although the analysis of steroids is complicated by the potential for many isomers, ion mobility spectrometry (IMS) has previously shown promise for the rapid separation of steroid isomers. This work is aimed at the further development of IMS separation for the analysis of steroids. Here, traveling wave ion mobility spectrometry (TWIMS) was applied to the study of group I metal adducted steroids and their corresponding multimers for five sets of isomers. Each set of isomers had a minimum of one dimeric metal ion adduct that exhibited a resolution greater than one (i.e., approaching baseline resolution). Additionally, ion-neutral collision cross sections (CCSs) were measured using polyalanine as a calibrant, which may provide an additional metric contributing to analyte identification. Where possible, measured CCSs were compared to previously reported values. When measuring CCSs of steroid isomers using polyalanine as the calibrant, nitrogen CCS values were within 1.0% error for monomeric sodiated adducts and slightly higher for the dimeric sodiated adducts. Overall, TWIMS was found to successfully separate steroids as dimeric adducts of group I metals. [Figure not available: see fulltext.].

Original languageEnglish (US)
Pages (from-to)248-255
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume30
Issue number2
DOIs
StatePublished - Feb 15 2019

Keywords

  • Collision cross section
  • Ion mobility spectrometry
  • Isomer discrimination
  • Metal ion adduction
  • Steroids

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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