Application of the peroxidase-antiperoxidase technique for the detection of enterotoxigenic Escherichia coli K99 pilus antigen in paraffin-embedded tissues

D. G. Rogers

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The peroxidase-antiperoxidase (PAP) technique was evaluated as a method for detecting enterotoxigenic Escherichia coli (ETEC) K99 pilus antigen in paraffin-embedded tissues of swine. Antigenic reactivity was optimal when tissues were processed in cold methanol according to a modified method of Sainte-Marie. This method of processing adequately preserved tissue morphology. The PAP technique was applicable to tissues where endogenous peroxidase activity was minimal.

Original languageEnglish (US)
Pages (from-to)762-766
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume33
Issue number8
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Anatomy
  • Histology

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