Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue

Scott A. Koepsell; Steven H. Hinrichs; Peter C. Iwen

doi:10.1128/JCM.01705-12

Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue

Scott A. Koepsell, Steven H. Hinrichs, Peter C. Iwen

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.

Original language	English (US)
Pages (from-to)	3395-3397
Number of pages	3
Journal	Journal of clinical microbiology
Volume	50
Issue number	10
DOIs	https://doi.org/10.1128/JCM.01705-12
State	Published - Oct 2012

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.01705-12

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title = "Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue",

abstract = "A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.",

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AU - Iwen, Peter C.

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AB - A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.

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Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue

Abstract

ASJC Scopus subject areas

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