Aspergillus niger mutants with increased glucose oxidase production

John Markwell, Laura G. Frakes, Eugene C. Brott, John Osterman, Fred W. Wagner

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Aspergillus niger NRRL-3, an organism used for the industrial scale production of d-gluconic acid and glucose oxidase (EC 1.1.3.4), was subjected to mutagenesis and selection for acid production on diagnostic media containing methyl red. The plates contained 0.1 M d-glucose, a concentration that does not produce a color change in the medium surrounding mycelia of the parental strain under the conditions employed. Mutagenized spores yielded occasional colonies which were able to grow rapidly and were surrounded by a reddish zone. A number of such presumptive mutants were selected and isolated. Twenty-six such strains were grown in shaken cultures with liquid media containing 0.01, 0.1 or 0.5 M d-glucose, harvested, disrupted and the specific activity of d-glucose oxidase determined. Seven of the mutant strains had glucose oxidase specific activities markedly higher than the parental strain.

Original languageEnglish (US)
Pages (from-to)166-169
Number of pages4
JournalApplied Microbiology and Biotechnology
Volume30
Issue number2
DOIs
StatePublished - Feb 1989

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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