Assay development for determination of tenofovir in human plasma by solid phase analytical derivatization and LC-MS/MS

Anthony T. Podany, Curtis Sheldon, Daryl Grafelman, Corey M. Ohnmacht

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

Background: A novel method was developed and validated to measure tenofovir in human plasma. Results/Methodology: This method employed solid phase analytical derivatization and analysis by LC-MS/MS. Stable-labeled internal standard was added to plasma samples followed by solid phase extraction. Retained analytes were derivatized on the solid phase extraction cartridges with a diazomethane solution to yield methyl-ester derivatives. Samples were analyzed using LC-MS/MS incorporating the use of a strong cation exchange column. The method was validated over a range of 5.00-750 ng/ml. The approach developed in this report for tenofovir could be applied to other analytes that share similar structural similarities. Conclusion: The tenofovir LC-MS/MS method was used to support a clinical study of over 400 samples with a 100% success rate.

Original languageEnglish (US)
Pages (from-to)3085-3095
Number of pages11
JournalBioanalysis
Volume7
Issue number24
DOIs
StatePublished - Dec 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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