Abstract
Background: A novel method was developed and validated to measure tenofovir in human plasma. Results/Methodology: This method employed solid phase analytical derivatization and analysis by LC-MS/MS. Stable-labeled internal standard was added to plasma samples followed by solid phase extraction. Retained analytes were derivatized on the solid phase extraction cartridges with a diazomethane solution to yield methyl-ester derivatives. Samples were analyzed using LC-MS/MS incorporating the use of a strong cation exchange column. The method was validated over a range of 5.00-750 ng/ml. The approach developed in this report for tenofovir could be applied to other analytes that share similar structural similarities. Conclusion: The tenofovir LC-MS/MS method was used to support a clinical study of over 400 samples with a 100% success rate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3085-3095 |
| Number of pages | 11 |
| Journal | Bioanalysis |
| Volume | 7 |
| Issue number | 24 |
| DOIs | |
| State | Published - Dec 2015 |
ASJC Scopus subject areas
- Analytical Chemistry
- General Pharmacology, Toxicology and Pharmaceutics
- Medical Laboratory Technology
- Clinical Biochemistry