An HPLC assay for etoposide in human serum was developed. Serum, spiked with podophyllotoxin (internal standard), was treated with sodium dodecyl sulphate prior to solid phase extraction. Analysis was performed on a 300 x 3.9 mm Bondclone 10 C18 column coupled with a fluorometric detector (λ(ex) 230 nm, λ(em) 330 nm). The retention times for etoposide and podophyllotoxin were 14 and 28 min respectively. The range of assay was 0.5 to 20 μg/ml with a detection limit of 0.2 μg/ml. This assay is suitable for use in clinical studies with etoposide.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Chromatography B: Biomedical Applications|
|State||Published - Apr 10 1998|
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