Assessing genetic diversity in the web blight pathogen Thanatephorus cucumeris (anamorph = Rhizoctonia solani) subgroups AG-1-IE and AG-1-IF with molecular markers

N. González, G. Godoy-Lutz, J. R. Steadman, R. Higgins, K. M. Eskridge

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Web blight, an important foliar disease of dry beans in the Americas, is a challenge to manage. We studied genetic variation of 92 isolates of Rhizoctonia solani subgroups AG-1-IE and AG-1-IF using DNA fingerprinting methods and mycelial compatibility grouping. The isolates were collected over 13 years from bean fields in the Dominican Republic, Honduras and Puerto Rico. Cluster and AMOVA analysis of combined data from two universal rice primers and two internal sequence repeats revealed significant genetic variation among and within populations of both subgroups. Variation was influenced by geographic origin and sampling year for AG-1-IE isolates and geographic origin for AG-1-IF isolates. Mycelial compatibility of paired isolates was mostly scored as incompatible in both subgroups and supported many unique phenotypes. Only two isolates of AG-1-IE displayed mycelial compatibility and DNA fingerprints, suggesting clonal origin. Genetic variation in these AG-1-IE and AG-1-IF isolate populations may explain the lack of durable resistance to web blight reported in dry beans.

Original languageEnglish (US)
Pages (from-to)85-98
Number of pages14
JournalJournal of General Plant Pathology
Volume78
Issue number2
DOIs
StatePublished - Mar 2012

Keywords

  • Anastomosis groups
  • DNA markers
  • DNA polymorphism
  • Phaseolus vulgaris
  • Population genetics

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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