Assessment of genotoxicity of nicotine employing in vitro mammalian test system

A. H. Trivedi; B. J. Dave; S. G. Adhvaryu

doi:10.1016/0304-3835(90)90096-G

Assessment of genotoxicity of nicotine employing in vitro mammalian test system

A. H. Trivedi, B. J. Dave, S. G. Adhvaryu

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Abstract

Genotoxicity of nicotine was evaluated employing Chinese hamster ovary (CHO) cells. Two cytogenetic endpoints, viz. frequency of sister chromatid exchange (SCE) and chromosome aberration (CA) were considered. Nicotine was found to induce CA and SCE frequency in a dose and duration dependent manner. Statistically significant elevations in CA frequency were observed only with higher concentrations of nicotine, whereas, SCE frequencies were increased significantly by all the doses utilized. It was genotoxic at the concentration, comparable to the saliva levels of nicotine achieved during tobacco chewing. The results obtained by continuous and pulse treatments with nicotine explain the harmful effects of chronic tobacco consumption.

Original language	English (US)
Pages (from-to)	89-94
Number of pages	6
Journal	Cancer Letters
Volume	54
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3835(90)90096-G
State	Published - Oct 8 1990
Externally published	Yes

Keywords

Chinese hamster ovary cells
chromosome aberration
genotoxicity
nicotine
sister chromatid exchange

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(90)90096-G

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abstract = "Genotoxicity of nicotine was evaluated employing Chinese hamster ovary (CHO) cells. Two cytogenetic endpoints, viz. frequency of sister chromatid exchange (SCE) and chromosome aberration (CA) were considered. Nicotine was found to induce CA and SCE frequency in a dose and duration dependent manner. Statistically significant elevations in CA frequency were observed only with higher concentrations of nicotine, whereas, SCE frequencies were increased significantly by all the doses utilized. It was genotoxic at the concentration, comparable to the saliva levels of nicotine achieved during tobacco chewing. The results obtained by continuous and pulse treatments with nicotine explain the harmful effects of chronic tobacco consumption.",

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AU - Trivedi, A. H.

AU - Dave, B. J.

AU - Adhvaryu, S. G.

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Y1 - 1990/10/8

N2 - Genotoxicity of nicotine was evaluated employing Chinese hamster ovary (CHO) cells. Two cytogenetic endpoints, viz. frequency of sister chromatid exchange (SCE) and chromosome aberration (CA) were considered. Nicotine was found to induce CA and SCE frequency in a dose and duration dependent manner. Statistically significant elevations in CA frequency were observed only with higher concentrations of nicotine, whereas, SCE frequencies were increased significantly by all the doses utilized. It was genotoxic at the concentration, comparable to the saliva levels of nicotine achieved during tobacco chewing. The results obtained by continuous and pulse treatments with nicotine explain the harmful effects of chronic tobacco consumption.

AB - Genotoxicity of nicotine was evaluated employing Chinese hamster ovary (CHO) cells. Two cytogenetic endpoints, viz. frequency of sister chromatid exchange (SCE) and chromosome aberration (CA) were considered. Nicotine was found to induce CA and SCE frequency in a dose and duration dependent manner. Statistically significant elevations in CA frequency were observed only with higher concentrations of nicotine, whereas, SCE frequencies were increased significantly by all the doses utilized. It was genotoxic at the concentration, comparable to the saliva levels of nicotine achieved during tobacco chewing. The results obtained by continuous and pulse treatments with nicotine explain the harmful effects of chronic tobacco consumption.

KW - Chinese hamster ovary cells

KW - chromosome aberration

KW - genotoxicity

KW - nicotine

KW - sister chromatid exchange

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Assessment of genotoxicity of nicotine employing in vitro mammalian test system

Abstract

Keywords

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