Association of a DNA marker with Hessian fly resistance gene H9 in wheat

I. Dweikat, H. Ohm, S. Mackenzie, F. Patterson, S. Cambron, R. Ratcliffe

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.

Original languageEnglish (US)
Pages (from-to)964-968
Number of pages5
JournalTheoretical and Applied Genetics
Volume89
Issue number7-8
DOIs
StatePublished - Dec 1 1994

Keywords

  • DNA markers
  • Hessian fly
  • RAPD
  • Wheat

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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    Dweikat, I., Ohm, H., Mackenzie, S., Patterson, F., Cambron, S., & Ratcliffe, R. (1994). Association of a DNA marker with Hessian fly resistance gene H9 in wheat. Theoretical and Applied Genetics, 89(7-8), 964-968. https://doi.org/10.1007/BF00224525