ATF-1 is activated in response to UV irradiation in B16 melanoma cells

Billie A. Moore, Robin Miskimins, W. Keith Miskimins

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Abstract

We have found that the transferrin receptor gene promoter is strongly activated, by exposure of B16 melanoma cells to UV light. This is a delayed event occurring more than 6 h after exposure and requires an AP-1/CRE-like element in the promoter as demonstrated by site-specific mutagenesis. UV irradiation enhances the binding of a nuclear factor to this element and supershift analysis demonstrates that this DNA-protein complex involves ATF-1. No other members of either the AP-1 or CREB/ATF families of transcription factors were found to bind to this DNA element in UV-irradiated B16 cells. Western blots show that the level of ATF-1 does not change following exposure to UV light, indicating that the increased binding of this factor is most likely mediated by posttranslational modifications in response to UV-mediated signaling pathways.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalMolecular Cell Biology Research Communications
Volume1
Issue number1
DOIs
StatePublished - Apr 1999

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ASJC Scopus subject areas

  • Molecular Biology

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