Atomic force microscopy investigation of a chlorella virus, PBCV-1

Yu G. Kuznetsov, James R. Gurnon, James L. Van Etten, Alexander McPherson

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A virus PBCV-1, which infects certain fresh water algae and has been shown by transmission and cryo-electron microscopy to exist as a triskaidecahedron, was imaged using atomic force microscopy (AFM). From AFM the particles have diameters of about 190 nm and the overall structure is in all important respects consistent with existing models. The surface lattice of the virion is composed of trimeric capsid proteins distributed according to p3 symmetry to create a honeycomb arrangement of raised edges forming quasi-hexagonal cells. At the pentagonal vertices are five copies of a different protein forming an exact pentagon, and this has yet another unique protein in its center. The apical protein exhibits some unusual mechanical properties in that it can be made to retract into the virion interior when subjected to AFM tip pressure. When PBCV-1 virions degrade, they give rise to small, uniform, spherical, and virus like particles (VLP) consistent with T = 1 or 3 icosahedral products. Also observed upon disintegration are strands of linear dsDNA. Fibers of unknown function are also occasionally seen associated with some virions.

Original languageEnglish (US)
Pages (from-to)256-263
Number of pages8
JournalJournal of Structural Biology
Volume149
Issue number3
DOIs
StatePublished - Mar 2005

Keywords

  • Algae
  • Degradation
  • Probe microscopy
  • Virus like particles
  • Virus structure

ASJC Scopus subject areas

  • Structural Biology

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