Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements

Yuri L. Lyubchenko; Bertram L. Jacobs; Stuart M. Lindsay

doi:10.1093/nar/20.15.3983

Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements

Yuri L. Lyubchenko, Bertram L. Jacobs, Stuart M. Lindsay

Research output: Contribution to journal › Article › peer-review

90 Scopus citations

Abstract

Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA olecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 #m. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 #n. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

Original language	English (US)
Pages (from-to)	3983-3986
Number of pages	4
Journal	Nucleic acids research
Volume	20
Issue number	15
DOIs	https://doi.org/10.1093/nar/20.15.3983
State	Published - Aug 11 1992
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{d8e71df6ff5941df8d265a10cd9f7c59,

title = "Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements",

abstract = "Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA olecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 #m. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 #n. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.",

author = "Lyubchenko, {Yuri L.} and Jacobs, {Bertram L.} and Lindsay, {Stuart M.}",

note = "Funding Information: We are grateful to L.Shlyakhtenko (University of Nevada Reno) for the discussion of the results and valuable criticism, U.Knipping (Arizona State University) for the help in image processing and R. Harrington (University of Nevada Reno) who provided support in addition to that described below. Financial support: research grants from NSF (DIR8920053) and ONR (N000149J1455) (SML and YLL) and grant CA48654 from the US Public Health Service (BLJ). Lyubchenko, Y.L. is on leave from the Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.",

year = "1992",

month = aug,

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doi = "10.1093/nar/20.15.3983",

language = "English (US)",

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journal = "Nucleic acids research",

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T1 - Atomic force microscopy of reovirus dsRNA

T2 - A routine technique for length measurements

AU - Lyubchenko, Yuri L.

AU - Jacobs, Bertram L.

AU - Lindsay, Stuart M.

N1 - Funding Information: We are grateful to L.Shlyakhtenko (University of Nevada Reno) for the discussion of the results and valuable criticism, U.Knipping (Arizona State University) for the help in image processing and R. Harrington (University of Nevada Reno) who provided support in addition to that described below. Financial support: research grants from NSF (DIR8920053) and ONR (N000149J1455) (SML and YLL) and grant CA48654 from the US Public Health Service (BLJ). Lyubchenko, Y.L. is on leave from the Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.

PY - 1992/8/11

Y1 - 1992/8/11

N2 - Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA olecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 #m. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 #n. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

AB - Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA olecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 #m. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 #n. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

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JO - Nucleic acids research

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IS - 15

ER -

Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements

Abstract

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