Atomic force microscopy of reovirus dsRNA: A routine technique for length measurements

Yuri L. Lyubchenko, Bertram L. Jacobs, Stuart M. Lindsay

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Atomic force microscopy (AFM) was used to image reovirus double stranded RNA (dsRNA) deposited from diluted buffer solution onto a chemically treated mica surface. This procedure allows AFM images of dsRNA olecules to be obtained with a quality close to that obtained with conventional electron microscopy. The length of the molecules were measured directly on a computer display using the digitally acquired images. The lengths of the molecules varied between 0.2 and 1.8 #m. Statistical analysis showed a multimodal distribution with clear maxima at 0.4, 0.65 and 1.05 #n. These data are in a good agreement with those obtained by electron microscopy and gel electrophoresis.

Original languageEnglish (US)
Pages (from-to)3983-3986
Number of pages4
JournalNucleic acids research
Volume20
Issue number15
DOIs
StatePublished - Aug 11 1992

ASJC Scopus subject areas

  • Genetics

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