Atrial natriuretic factor inhibits angiotensin-induced aldosterone secretion: Not through cGMP or interference with phospholipase C

ANF did not prevent the formation of [³H] inositol trisphosphate in response to All but inhibited aldosterone secretion in calf adrenal glomerulosa cells. 8-bromo cGMP did not affect either inositol phosphate formation or aldosterone secretion. Changes in cytosolic Ca⁺⁺ concentration induced by All, as measured by Quin 2 fluorescence, were also unaffected by ANF. No difference in adrenal cell protein phosphorylation with All or All+ANF was observed. The results suggest that ANF may inhibit aldosterone secretion through a non-guanyl cyclase linked receptor system not involving the formation of phosphoinositide-derived second messengers. Interference with protein kinase C activity cannot be ruled out.