Augmentation of natural cytotoxicity by leucine enkephalin in cultured peripheral blood mononuclear cells from patients infected with human immunodeficiency virus

Dorothy Oleson; Helen Grierson; Jonathan Goldsmith; David T. Purtilo; Donald Johnson

doi:10.1016/0090-1229(89)90036-6

Augmentation of natural cytotoxicity by leucine enkephalin in cultured peripheral blood mononuclear cells from patients infected with human immunodeficiency virus

Dorothy Oleson, Helen Grierson, Jonathan Goldsmith, David T. Purtilo, Donald Johnson

Pathology & Microbiology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Natural Killer (NK) activity of lymphocytes from acquired immunodeficiency syndrome (AIDS) patients is frequently below normal and declines as disease progresses. We studied the potential of leucine enkephalin (leu-enkephalin) to restore this immune parameter by incubating nylon wool nonadherent mononuclear cells from 14 patients in the presence or absence of leu-enkephalin, and measuring NK cytolysis in a standard ⁵¹Cr release assay. The NK activity of human immunodeficiency virus antibody positive (HIV⁺) individuals with some remaining NK lytic ability was significantly augmented by leu-enkephalin concentrations of 10^-10 and 10^-8 M (n = 7). HIV⁺ patients with no measurable basal level of NK activity (n = 3) were not responsive to stimulation with leu-enkephalin. Human immunodeficiency virus antibody negative (HIV^-) individuals (n = 4) responded in a pattern similar to normals. In addition, naloxone, an antagonist of alkaloid and peptide opiates including leu-enkephalin, displayed the properties of an antagonist/agonist, reflecting the immunoregulatory capacities of the endogenous opiate system.

Original language	English (US)
Pages (from-to)	386-395
Number of pages	10
Journal	Clinical Immunology and Immunopathology
Volume	51
Issue number	3
DOIs	https://doi.org/10.1016/0090-1229(89)90036-6
State	Published - Mar 1989

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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Augmentation of natural cytotoxicity by leucine enkephalin in cultured peripheral blood mononuclear cells from patients infected with human immunodeficiency virus. / Oleson, Dorothy; Grierson, Helen; Goldsmith, Jonathan et al.
In: Clinical Immunology and Immunopathology, Vol. 51, No. 3, 03.1989, p. 386-395.

Research output: Contribution to journal › Article › peer-review

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title = "Augmentation of natural cytotoxicity by leucine enkephalin in cultured peripheral blood mononuclear cells from patients infected with human immunodeficiency virus",

abstract = "Natural Killer (NK) activity of lymphocytes from acquired immunodeficiency syndrome (AIDS) patients is frequently below normal and declines as disease progresses. We studied the potential of leucine enkephalin (leu-enkephalin) to restore this immune parameter by incubating nylon wool nonadherent mononuclear cells from 14 patients in the presence or absence of leu-enkephalin, and measuring NK cytolysis in a standard 51Cr release assay. The NK activity of human immunodeficiency virus antibody positive (HIV+) individuals with some remaining NK lytic ability was significantly augmented by leu-enkephalin concentrations of 10-10 and 10-8 M (n = 7). HIV+ patients with no measurable basal level of NK activity (n = 3) were not responsive to stimulation with leu-enkephalin. Human immunodeficiency virus antibody negative (HIV-) individuals (n = 4) responded in a pattern similar to normals. In addition, naloxone, an antagonist of alkaloid and peptide opiates including leu-enkephalin, displayed the properties of an antagonist/agonist, reflecting the immunoregulatory capacities of the endogenous opiate system.",

author = "Dorothy Oleson and Helen Grierson and Jonathan Goldsmith and Purtilo, {David T.} and Donald Johnson",

note = "Funding Information: This work was supported by NIH Grant CA-32%9, PHS Grant MCI-192002, and the Imperial Court of Nebraska. Dorothy Oleson is a recipient of a Presidential Graduate Fellowship. We thank Marge Boyden for expert preparation of this manuscript.",

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AU - Johnson, Donald

N1 - Funding Information: This work was supported by NIH Grant CA-32%9, PHS Grant MCI-192002, and the Imperial Court of Nebraska. Dorothy Oleson is a recipient of a Presidential Graduate Fellowship. We thank Marge Boyden for expert preparation of this manuscript.

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N2 - Natural Killer (NK) activity of lymphocytes from acquired immunodeficiency syndrome (AIDS) patients is frequently below normal and declines as disease progresses. We studied the potential of leucine enkephalin (leu-enkephalin) to restore this immune parameter by incubating nylon wool nonadherent mononuclear cells from 14 patients in the presence or absence of leu-enkephalin, and measuring NK cytolysis in a standard 51Cr release assay. The NK activity of human immunodeficiency virus antibody positive (HIV+) individuals with some remaining NK lytic ability was significantly augmented by leu-enkephalin concentrations of 10-10 and 10-8 M (n = 7). HIV+ patients with no measurable basal level of NK activity (n = 3) were not responsive to stimulation with leu-enkephalin. Human immunodeficiency virus antibody negative (HIV-) individuals (n = 4) responded in a pattern similar to normals. In addition, naloxone, an antagonist of alkaloid and peptide opiates including leu-enkephalin, displayed the properties of an antagonist/agonist, reflecting the immunoregulatory capacities of the endogenous opiate system.

AB - Natural Killer (NK) activity of lymphocytes from acquired immunodeficiency syndrome (AIDS) patients is frequently below normal and declines as disease progresses. We studied the potential of leucine enkephalin (leu-enkephalin) to restore this immune parameter by incubating nylon wool nonadherent mononuclear cells from 14 patients in the presence or absence of leu-enkephalin, and measuring NK cytolysis in a standard 51Cr release assay. The NK activity of human immunodeficiency virus antibody positive (HIV+) individuals with some remaining NK lytic ability was significantly augmented by leu-enkephalin concentrations of 10-10 and 10-8 M (n = 7). HIV+ patients with no measurable basal level of NK activity (n = 3) were not responsive to stimulation with leu-enkephalin. Human immunodeficiency virus antibody negative (HIV-) individuals (n = 4) responded in a pattern similar to normals. In addition, naloxone, an antagonist of alkaloid and peptide opiates including leu-enkephalin, displayed the properties of an antagonist/agonist, reflecting the immunoregulatory capacities of the endogenous opiate system.

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Augmentation of natural cytotoxicity by leucine enkephalin in cultured peripheral blood mononuclear cells from patients infected with human immunodeficiency virus

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