Automated determination of β-galactosidase specific activity

P. R. Bianco, G. M. Weinstock

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We describe a modification of an automated kinetic assay for β- galactosidase (β-gal) activity. This modification includes an assay to quantitate the amount of protein added to each assay. The determination of specific activity includes the amount of protein in the calculation, which produces a specific activity with units of pmol product produced/minute/mg protein. In addition to this modification, we present a series of macros written in Microsoft® Excel(TM) for either the Macintosh® or Windows(TM) on the PC. These macros decrease the amount of time required to analyze the data from β-gal assays.

Original languageEnglish (US)
Pages (from-to)974-976+978
JournalBioTechniques
Volume17
Issue number5
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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