Basic fibroblast growth factor and growth factor receptor gene expression in 85% O2-exposed rat lung

S. Buch, R. N.N. Han, J. Liu, A. Moore, J. D. Edelson, B. A. Freeman, M. Post, A. K. Tanswell

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Lungs exposed to elevated O2 concentrations suffer an initial loss of type I pneumocytes, followed by a reparative type II pneumocyte hyperplasia. We hypothesized that type II pneumocyte hyperplasia after exposure of young adult rats to 85% O2 in vivo would be temporally related to 1) an increased concentration of intrapulmonary basic fibroblast growth factor (bFGF), a potent stimulator of type II pneumocyte DNA synthesis in vitro, and 2) an upregulation of pneumocyte receptors for bFGF (FGF-R). Increased rat lung bFGF mRNA, relative to air-exposed control animals, was observed at 4 days of exposure, with no increase at days 6 and 14 of exposure. Parallel changes were observed with bFGF receptor (flg) mRNA. Nuclear runoff assays confirmed increased transcription of both bFGF and flg genes in response to 85% O2, whereas increased translation at 6 days of exposure was confirmed by protein immunoanalysis. Immunohistochemistry demonstrated a broad distribution of bFGF throughout the lung, including the alveolar epithelium, which increased after 6 and 14 days of exposure to 85% O2. Our findings are compatible with a role for bFGF in O2-mediated pneumocyte hyperplasia.

Original languageEnglish (US)
Pages (from-to)L455-L464
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume268
Issue number3 12-3
DOIs
StatePublished - Jan 1 1995

Keywords

  • fibroblast growth factor receptor
  • flg
  • pneumocyte hyperplasia
  • pulmonary oxygen toxicity
  • type II pneumocyte

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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