Abstract
Affinity labeling of iodinated neuropeptide Y (NPY) to bovine hippocampal binding proteins revealed that benextramine inhibited specific NPY labeling of the 50 kDa NPY binding protein (Y2 binding protein) in a dose-dependent manner (IC50 = 33 M). Hippocampal membranes, which were pretreated with benextramine and washed, exhibited decreased [125I]NPY labeling of binding proteins in a similar dose-dependent manner. These findings demonstrate that benextramine irreversibly blocks specific NPY binding to the 50 kDa NPY Y2 binding protein.
Original language | English (US) |
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Pages (from-to) | 89-91 |
Number of pages | 3 |
Journal | European Journal of Pharmacology: Molecular Pharmacology |
Volume | 207 |
Issue number | 1 |
DOIs | |
State | Published - May 25 1991 |
Keywords
- Affinity labeling
- Benextramine
- Hippocampus (bovine)
- Neuropeptide Y (NPY)
- Y binding protein
ASJC Scopus subject areas
- Pharmacology