Binding of actinomycin D to single-stranded DNA

Elizabeth A. Jares-Erijman; Reinhard Klement; Reinhard Machinek; Randy M. Wadkins; Besik I. Kankia; Luis A. Marky; Thomas M. Jovin

doi:10.1080/07328319708002931

Binding of actinomycin D to single-stranded DNA

Elizabeth A. Jares-Erijman, Reinhard Klement, Reinhard Machinek, Randy M. Wadkins, Besik I. Kankia, Luis A. Marky, Thomas M. Jovin

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The sequence specificity and structural aspects of the mode of interaction of the antitumor drug actinomycin D (AMD) with single-stranded DNA were studied by fluorescence, absorption and NMR spectroscopy, calorimetry, ultrasonic velocity and density measurements, and molecular modeling. The binding is length and sequence dependent, with the tetranucleotide motif TAGT showing the highest affinity. A 'hemi- intercalation' model for the interaction is proposed.

Original language	English (US)
Pages (from-to)	661-667
Number of pages	7
Journal	Nucleosides and Nucleotides
Volume	16
Issue number	5-6
DOIs	https://doi.org/10.1080/07328319708002931
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Genetics

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abstract = "The sequence specificity and structural aspects of the mode of interaction of the antitumor drug actinomycin D (AMD) with single-stranded DNA were studied by fluorescence, absorption and NMR spectroscopy, calorimetry, ultrasonic velocity and density measurements, and molecular modeling. The binding is length and sequence dependent, with the tetranucleotide motif TAGT showing the highest affinity. A 'hemi- intercalation' model for the interaction is proposed.",

author = "Jares-Erijman, {Elizabeth A.} and Reinhard Klement and Reinhard Machinek and Wadkins, {Randy M.} and Kankia, {Besik I.} and Marky, {Luis A.} and Jovin, {Thomas M.}",

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AU - Jares-Erijman, Elizabeth A.

AU - Klement, Reinhard

AU - Machinek, Reinhard

AU - Wadkins, Randy M.

AU - Kankia, Besik I.

AU - Marky, Luis A.

AU - Jovin, Thomas M.

PY - 1997

Y1 - 1997

N2 - The sequence specificity and structural aspects of the mode of interaction of the antitumor drug actinomycin D (AMD) with single-stranded DNA were studied by fluorescence, absorption and NMR spectroscopy, calorimetry, ultrasonic velocity and density measurements, and molecular modeling. The binding is length and sequence dependent, with the tetranucleotide motif TAGT showing the highest affinity. A 'hemi- intercalation' model for the interaction is proposed.

AB - The sequence specificity and structural aspects of the mode of interaction of the antitumor drug actinomycin D (AMD) with single-stranded DNA were studied by fluorescence, absorption and NMR spectroscopy, calorimetry, ultrasonic velocity and density measurements, and molecular modeling. The binding is length and sequence dependent, with the tetranucleotide motif TAGT showing the highest affinity. A 'hemi- intercalation' model for the interaction is proposed.

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SN - 0732-8311

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ER -

Binding of actinomycin D to single-stranded DNA

Abstract

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