Binding of actinomycin D to single-stranded DNA

Elizabeth A. Jares-Erijman, Reinhard Klement, Reinhard Machinek, Randy M. Wadkins, Besik I. Kankia, Luis A. Marky, Thomas M. Jovin

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The sequence specificity and structural aspects of the mode of interaction of the antitumor drug actinomycin D (AMD) with single-stranded DNA were studied by fluorescence, absorption and NMR spectroscopy, calorimetry, ultrasonic velocity and density measurements, and molecular modeling. The binding is length and sequence dependent, with the tetranucleotide motif TAGT showing the highest affinity. A 'hemi- intercalation' model for the interaction is proposed.

Original languageEnglish (US)
Pages (from-to)661-667
Number of pages7
JournalNucleosides and Nucleotides
Issue number5-6
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Genetics


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