@article{a15f9f925bb24a189aa049516a3a67d3,
title = "Bioactivity of recombinant hFSH glycosylation variants in primary cultures of porcine granulosa cells",
abstract = "Previous studies have reported hypo-glycosylated FSH and fully-glycosylated FSH to be naturally occurring in humans, and these glycoforms exist in changing ratios over a woman's lifespan. The precise cellular and molecular effects of recombinant human FSH (hFSH) glycoforms, FSH21 and FSH24, have not been documented in primary granulosa cells. Herein, biological responses to FSH21 and FSH24 were compared in primary porcine granulosa cells. Hypo-glycosylated hFSH21 was significantly more effective than fully-glycosylated hFSH24 at stimulating cAMP accumulation and protein kinase A (PKA) activity, leading to the higher phosphorylation of CREB and β-Catenin. Compared to fully-glycosylated hFSH24, hypo-glycosylated hFSH21 also induced greater levels of transcripts for HSD3B, STAR and INHA, and higher progesterone production. Our results demonstrate that hypo-glycosylated hFSH21 exerts more robust activation of intracellular signals associated with steroidogenesis than fully-glycosylated hFSH24 in primary porcine granulosa cells, and furthers our understanding of the differing bioactivities of FSH glycoforms in the ovary.",
keywords = "Cell signaling, Follicle, Ovary, Protein kinase A, Steroidogenesis, β-Catenin",
author = "Aixin Liang and Plewes, {Michele R.} and Guohua Hua and Xiaoying Hou and Blum, {Haley R.} and Emilia Przygrodzka and George, {Jitu W.} and Clark, {Kendra L.} and Bousfield, {George R.} and Butnev, {Viktor Y.} and May, {Jeffrey V.} and Davis, {John S.}",
note = "Funding Information: This work was supported by the National Institutes of Health [ 1P01 AG029531 ], and the Olson Center for Women's Health . This work was supported in part by a Senior Research Career Scientist Award and Merit Review Award I01 BX004272 from the United States Department of Veterans Affairs Biomedical Laboratory Research and Development Service. This material is the result of work supported with resources and the use of facilities at the VA NWIHCS, Omaha, NE. Funding Information: This work was supported by the National Institutes of Health [1P01 AG029531], and the Olson Center for Women's Health. This work was supported in part by a Senior Research Career Scientist Award and Merit Review Award I01 BX004272 from the United States Department of Veterans Affairs Biomedical Laboratory Research and Development Service. This material is the result of work supported with resources and the use of facilities at the VA NWIHCS, Omaha, NE. Funding Information: The authors thank advanced Microscopy Core Facility for their assistance with microscopy. The use of the microscope was supported by Center for Cellular Signaling CoBRE-P30GM106397 from the National Health Institutes. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = aug,
day = "20",
doi = "10.1016/j.mce.2020.110911",
language = "English (US)",
volume = "514",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
}