Bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads

Danny J. Schnell; Mary Ann Pfenstiel; Kenneth W. Nickerson

doi:10.1126/science.6701520

Bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads

Danny J. Schnell, Mary Ann Pfenstiel, Kenneth W. Nickerson

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Solubilized crystals of Bacillus thuringiensis var. israelensis were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.

Original language	English (US)
Pages (from-to)	1193-1195
Number of pages	3
Journal	Science
Volume	223
Issue number	4641
DOIs	https://doi.org/10.1126/science.6701520
State	Published - 1984
Externally published	Yes

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title = "Bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads",

abstract = "Solubilized crystals of Bacillus thuringiensis var. israelensis were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.",

author = "Schnell, {Danny J.} and Pfenstiel, {Mary Ann} and Nickerson, {Kenneth W.}",

year = "1984",

doi = "10.1126/science.6701520",

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journal = "Science",

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publisher = "American Association for the Advancement of Science",

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T1 - Bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads

AU - Schnell, Danny J.

AU - Pfenstiel, Mary Ann

AU - Nickerson, Kenneth W.

PY - 1984

Y1 - 1984

N2 - Solubilized crystals of Bacillus thuringiensis var. israelensis were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.

AB - Solubilized crystals of Bacillus thuringiensis var. israelensis were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.

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U2 - 10.1126/science.6701520

DO - 10.1126/science.6701520

M3 - Article

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JO - Science

JF - Science

IS - 4641

ER -

Bioassay of solubilized bacillus thuringiensis var. israelensis crystals by attachment to latex beads

Abstract

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