TY - JOUR
T1 - Bioassays of compounds with potential juvenoid activity on Drosophila melanogaster
T2 - Juvenile hormone III, bisepoxide juvenile hormone III and methyl farnesoates
AU - Harshman, Lawrence G.
AU - Song, Ki Duck
AU - Casas, Josephina
AU - Schuurmans, A.
AU - Kuwano, Eichii
AU - Kachman, Stephen D.
AU - Riddiford, Lynn M.
AU - Hammock, Bruce D.
N1 - Funding Information:
Appreciation is expressed to Grace and Davey Jones, University of Kentucky, who are interested in the potential role of methyl farnesoate as a compound with juvenile hormone-like activity in D. melanogaster. They encouraged the publication of the present study which is based on a manuscript that was written approximately 15 years ago. This research was supported by USDA (Competitive Research Grants Program 2007-35607-17830 ) and NIH ( RO1ES0072710 ) grants to BDH and a NSF grant to LMR. LGH is currently supported by NIH ( RO1 DK074136 ) and is a member of the Nebraska Gateway for Nutritional Genomics group at the University of Nebraska-Lincoln.
PY - 2010/10
Y1 - 2010/10
N2 - Metabolites of the 6,7,10,11 bisepoxide juvenile hormone III (JHB3), and other potential juvenoids, were tested for juvenile hormone activity using early instar or early stage pupae of Drosophila melanogaster. Importantly, methyl farnesoates were tested as they might have JH-like activity on Dipteran juveniles. Larvae were exposed to compounds in medium, or the compounds were applied to white puparia. In the assays employed in the present study, there was no indication for JH activity associated with the metabolites of JHB3. The activity of methyl farnesoate (MF) was higher than that of JH III and far greater than bisepoxide JH III. As opposed to the two endogenous juvenile hormones, methyl farnesoate has weak activity in the white puparial bioassay. When fluorinated forms of methyl farnesoate, which is unlikely to be converted to JH, were applied to Drosophila medium to which fly eggs were introduced, there was a high degree of larval mortality, but no evidence of subsequent mortality at the pupal stage. One possible explanation for the results is that methyl farnesoate is active as a hormone in larval stages, but has little activity at the pupal stage where only juvenile hormone has a major effect.
AB - Metabolites of the 6,7,10,11 bisepoxide juvenile hormone III (JHB3), and other potential juvenoids, were tested for juvenile hormone activity using early instar or early stage pupae of Drosophila melanogaster. Importantly, methyl farnesoates were tested as they might have JH-like activity on Dipteran juveniles. Larvae were exposed to compounds in medium, or the compounds were applied to white puparia. In the assays employed in the present study, there was no indication for JH activity associated with the metabolites of JHB3. The activity of methyl farnesoate (MF) was higher than that of JH III and far greater than bisepoxide JH III. As opposed to the two endogenous juvenile hormones, methyl farnesoate has weak activity in the white puparial bioassay. When fluorinated forms of methyl farnesoate, which is unlikely to be converted to JH, were applied to Drosophila medium to which fly eggs were introduced, there was a high degree of larval mortality, but no evidence of subsequent mortality at the pupal stage. One possible explanation for the results is that methyl farnesoate is active as a hormone in larval stages, but has little activity at the pupal stage where only juvenile hormone has a major effect.
KW - Bioassay
KW - Bisepoxide juvenile hormone
KW - Drosophila
KW - Juvenile hormone
KW - Methyl farnesoate
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U2 - 10.1016/j.jinsphys.2010.06.003
DO - 10.1016/j.jinsphys.2010.06.003
M3 - Article
C2 - 20599543
AN - SCOPUS:77955424091
SN - 0022-1910
VL - 56
SP - 1465
EP - 1470
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
IS - 10
ER -