Biochemistry and ultrastructure of iridescent virus type 29

Paul N. Black, Carol D. Blair, Ann Butcher, John L. Capinera, George M. Happ

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Physical and chemical parameters of iridescent virus type 29, isolated from the mealworm, Tenebrio molitor, have been analyzed. The icosahedral capsid is 130-135 nm in diameter and is surrounded by a fringe of coarse filaments. The virus has a buoyant density in CsCl of 1.31 g cm-3 and contains 20 to 25 structural proteins as analyzed by isoelectric focusing and SDS-polyacrylamide gel electrophoresis. The DNA has a buoyant density in CsCl of 1.6874 g cm-3 indicating a G + C content of approximately 28%. The lipid components of this virus differ from those of the host cell; the virus contains about 80% cardiolipin and 20% phosphatidyl choline.

Original languageEnglish (US)
Pages (from-to)12-21
Number of pages10
JournalJournal of Invertebrate Pathology
Volume38
Issue number1
DOIs
StatePublished - Jul 1981

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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