TY - JOUR
T1 - Biodegradable polyanhydride-based nanomedicines for blood to brain drug delivery
AU - Brenza, Timothy M.
AU - Schlichtmann, Benjamin W.
AU - Bhargavan, Biju
AU - Vela Ramirez, Julia E.
AU - Nelson, Rainie D.
AU - Panthani, Matthew G.
AU - McMillan, Jo Ellyn M.
AU - Kalyanaraman, Balaraman
AU - Gendelman, Howard E.
AU - Anantharam, Vellareddy
AU - Kanthasamy, Anumantha G.
AU - Mallapragada, Surya K.
AU - Narasimhan, Balaji
AU - Kanmogne, Georgette D.
N1 - Funding Information:
Balaji Narasimhan and Georgette D. Kanmogne shared corresponding authors pending manuscript acceptance. Correspondence to: G. D. Kanmogne; e-mail: gkanmogne@unmc.edu or B. Narasimhan; e-mail: nbalaji@iastate.edu Contract grant sponsor: US Army Medical Research and Materiel Command; contract grant number: W81XWH-11–1-0700 Contract grant sponsor: Iowa State University Nanovaccine Institute Contract grant sponsor: National Institute of Health grants; contract grant number: R01 MH081780 and R01 MH094160 and P01DA028555 and R01 AG043530 and P30 MH062261 and R01 MH115860 and R01 NS034249 and R01 NS036126 Contract grant sponsor: National Science Foundation Graduate Research Fellowship Program; contract grant number: DGE1247194 (RDN)
PY - 2018/11
Y1 - 2018/11
N2 - An urgent need to deliver therapeutics across the blood–brain barrier (BBB) underlies a paucity of effective therapies currently available for treatment of degenerative, infectious, traumatic, chemical, and metabolic disorders of the nervous system. With an eye toward achieving this goal, an in vitro BBB model was employed to simulate biodegradable polyanhydride nanoparticle-based drug delivery to the brain. Using a combination of confocal microscopy, flow cytometry, and high performance liquid chromatography, we examined the potential of polyanhydride nanoparticles containing the anti-oxidant, mito-apocynin, to be internalized and then transferred from monocytes to human brain microvascular endothelial cells. The efficacy of this nanoparticle-based delivery platform was demonstrated by neuronal protection against oxidative stress. Taken together, this polyanhydride nanoparticle-based delivery system holds promise for enhancing neuroprotection by facilitating drug transport across the BBB.
AB - An urgent need to deliver therapeutics across the blood–brain barrier (BBB) underlies a paucity of effective therapies currently available for treatment of degenerative, infectious, traumatic, chemical, and metabolic disorders of the nervous system. With an eye toward achieving this goal, an in vitro BBB model was employed to simulate biodegradable polyanhydride nanoparticle-based drug delivery to the brain. Using a combination of confocal microscopy, flow cytometry, and high performance liquid chromatography, we examined the potential of polyanhydride nanoparticles containing the anti-oxidant, mito-apocynin, to be internalized and then transferred from monocytes to human brain microvascular endothelial cells. The efficacy of this nanoparticle-based delivery platform was demonstrated by neuronal protection against oxidative stress. Taken together, this polyanhydride nanoparticle-based delivery system holds promise for enhancing neuroprotection by facilitating drug transport across the BBB.
KW - blood–brain barrier
KW - cell-mediated transcytosis
KW - mito-apocynin
KW - nanoparticles
KW - polyanhydrides
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U2 - 10.1002/jbm.a.36477
DO - 10.1002/jbm.a.36477
M3 - Article
C2 - 30369055
AN - SCOPUS:85055703769
VL - 106
SP - 2881
EP - 2890
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 1549-3296
IS - 11
ER -