Biomarkers in diagnosis of pancreatic carcinoma in fine-needle aspirates: A translational research application

Nirag Jhala, Darshana Jhala, Selwyn M. Vickers, Isam Eltoum, Surinder K. Batra, Upender Manne, Mohamad Eloubeidi, Jennifer J. Jones, William E. Grizzle

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

This study was undertaken to determine whether recently identified proteins could be translated to clinical practice as markers to distinguish pancreatic adenocarcinoma from chronic pancreatitis on fine-needle aspirate (FNA) samples. Resected pancreatic tissue sections (n = 40) and FNA samples (n = 65) were stained for clusterin-β, MUC4, survivin, and mesothelin. For each biomarker, the staining patterns in adenocarcinoma and in reactive ductal epithelium were evaluated and compared. Clusterin-β stained reactive ductal epithelium significantly more frequently than pancreatic adenocarcinoma (P < .001). In comparison, MUC4 and mesothelin were expressed more frequently in pancreatic adenocarcinoma on tissue sections. Positive staining for MUC4 (91% vs 0%; P < .001) and mesothelin (62% vs 0%; P = .01) and absence of staining for clusterin-β (90% vs 7%; P < .001) were noted significantly more frequently in adenocarcinoma cells than in reactive cells in FNA samples. Clusterin-β and MUC4 can help distinguish reactive ductal epithelial cells from the cells of pancreatic adenocarcinoma in FNA samples.

Original languageEnglish (US)
Pages (from-to)572-579
Number of pages8
JournalAmerican journal of clinical pathology
Volume126
Issue number4
DOIs
StatePublished - Oct 2006

Keywords

  • Biological markers
  • Cytology
  • Cytopathology
  • Endoscopic ultrasound
  • Fine-needle aspiration
  • Gastrointestinal
  • Pancreatic carcinoma
  • Translational research

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Jhala, N., Jhala, D., Vickers, S. M., Eltoum, I., Batra, S. K., Manne, U., Eloubeidi, M., Jones, J. J., & Grizzle, W. E. (2006). Biomarkers in diagnosis of pancreatic carcinoma in fine-needle aspirates: A translational research application. American journal of clinical pathology, 126(4), 572-579. https://doi.org/10.1309/CEV30BE088CBDQD9