TY - JOUR
T1 - Bioprinted Injectable Hierarchically Porous Gelatin Methacryloyl Hydrogel Constructs with Shape-Memory Properties
AU - Ying, Guoliang
AU - Jiang, Nan
AU - Parra-Cantu, Carolina
AU - Tang, Guosheng
AU - Zhang, Jingyi
AU - Wang, Hongjun
AU - Chen, Shixuan
AU - Huang, Ning Ping
AU - Xie, Jingwei
AU - Zhang, Yu Shrike
N1 - Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/11/11
Y1 - 2020/11/11
N2 - Direct injection of cell-laden hydrogels shows high potential for tissue regeneration in translational therapy. The traditional cell-laden hydrogels are often used as bulk space fillers to tissue defects after injection, likely limiting their structural controllability. On the other hand, patterned cell-laden hydrogel constructs often necessitate invasive surgical procedures. To overcome these problems, herein, a unique strategy is reported for encapsulating living human cells in a pore-forming gelatin methacryloyl (GelMA)-based bioink to ultimately produce injectable hierarchically macro-micro-nanoporous cell-laden GelMA hydrogel constructs through 3D extrusion bioprinting. The hydrogel constructs can be fabricated into various shapes and sizes that are defect-specific. Due to the hierarchically macro-micro-nanoporous structures, the cell-laden hydrogel constructs can readily recover to their original shapes, and sustain high cell viability, proliferation, spreading, and differentiation after compression and injection. In addition, in vivo studies further reveal that the hydrogel constructs can integrate well with the surrounding host tissues. These findings suggest that the unique 3D-bioprinted pore-forming GelMA hydrogel constructs are promising candidates for applications in minimally invasive tissue regeneration and cell therapy.
AB - Direct injection of cell-laden hydrogels shows high potential for tissue regeneration in translational therapy. The traditional cell-laden hydrogels are often used as bulk space fillers to tissue defects after injection, likely limiting their structural controllability. On the other hand, patterned cell-laden hydrogel constructs often necessitate invasive surgical procedures. To overcome these problems, herein, a unique strategy is reported for encapsulating living human cells in a pore-forming gelatin methacryloyl (GelMA)-based bioink to ultimately produce injectable hierarchically macro-micro-nanoporous cell-laden GelMA hydrogel constructs through 3D extrusion bioprinting. The hydrogel constructs can be fabricated into various shapes and sizes that are defect-specific. Due to the hierarchically macro-micro-nanoporous structures, the cell-laden hydrogel constructs can readily recover to their original shapes, and sustain high cell viability, proliferation, spreading, and differentiation after compression and injection. In addition, in vivo studies further reveal that the hydrogel constructs can integrate well with the surrounding host tissues. These findings suggest that the unique 3D-bioprinted pore-forming GelMA hydrogel constructs are promising candidates for applications in minimally invasive tissue regeneration and cell therapy.
KW - 3D bioprinting
KW - injectable cell-laden hydrogels
KW - pore-forming hydrogels
KW - shape-memory hydrogels
KW - tissue repair
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U2 - 10.1002/adfm.202003740
DO - 10.1002/adfm.202003740
M3 - Article
C2 - 33708030
AN - SCOPUS:85090215643
SN - 1616-301X
VL - 30
JO - Advanced Functional Materials
JF - Advanced Functional Materials
IS - 46
M1 - 2003740
ER -