Biosynthesis of glucosinolates in the developing silique walls and seeds of Sinapis alba

Liangcheng Du, Barbara Ann Halkier

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

When [14C]tyrosine was administered to developing seeds and silique walls of Sinapis alba L. both tissues de novo synthesized p- hydroxybenzylglucosinolate (p-OHBG). Within a silique, the incorporation rate of tyrosine to p-OHBG was up to 70-fold higher in the silique wails compared to the seeds as evidenced by both in vivo and in vitro measurements. During development, the amount of p-OHBG increases in the seeds, whereas the level remains relatively constant in the corresponding walls in radioisotope feeding experiments using intact siliques. This shows that p-OHBG is translocated from the walls to the seeds in S. alba. Although translocation is a major contributor to glucosinolate accumulation in the seeds, we also demonstrate that seeds of S. alba contain all the enzymes required for de novo biosynthesis of p-OHBG.

Original languageEnglish (US)
Pages (from-to)1145-1150
Number of pages6
JournalPhytochemistry
Volume48
Issue number7
DOIs
StatePublished - Aug 1998

Keywords

  • Biosynthesis
  • Brassicaceae
  • Developing silique
  • Glucosinolates
  • Sinapis alba

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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