TY - JOUR
T1 - Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli
AU - Tong, Wenhua
AU - Xu, Ying
AU - Xian, Mo
AU - Niu, Wei
AU - Guo, Jiantao
AU - Liu, Huizhou
AU - Zhao, Guang
N1 - Funding Information:
This work was supported by the 100-Talent Project of CAS (for GZ), CAS Key Program (KGZD-EW-606-1-3), and Taishan Scholars Climbing Program of Shandong (No.TSPD20150210).
Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/6
Y1 - 2016/6
N2 - Acrylic acid is an important industrial feedstock. In this study, a de novo acrylate biosynthetic pathway from inexpensive carbon source glycerol was constructed in Escherichia coli. The acrylic acid was produced from glycerol via 3-hydroxypropionaldehyde, 3-hydroxypropionyl-CoA, and acrylyl-CoA. The acrylate production was improved by screening and site-directed mutagenesis of key enzyme enoyl-CoA hydratase and chromosomal integration of some exogenous genes. Finally, our recombinant strain produced 37.7 mg/L acrylic acid under shaking flask conditions. Although the acrylate production is low, our study shows feasibility of engineering an acrylate biosynthetic pathway from inexpensive carbon source. Furthermore, the reasons for limited acrylate production and further strain optimization that should be performed in the future were also discussed.
AB - Acrylic acid is an important industrial feedstock. In this study, a de novo acrylate biosynthetic pathway from inexpensive carbon source glycerol was constructed in Escherichia coli. The acrylic acid was produced from glycerol via 3-hydroxypropionaldehyde, 3-hydroxypropionyl-CoA, and acrylyl-CoA. The acrylate production was improved by screening and site-directed mutagenesis of key enzyme enoyl-CoA hydratase and chromosomal integration of some exogenous genes. Finally, our recombinant strain produced 37.7 mg/L acrylic acid under shaking flask conditions. Although the acrylate production is low, our study shows feasibility of engineering an acrylate biosynthetic pathway from inexpensive carbon source. Furthermore, the reasons for limited acrylate production and further strain optimization that should be performed in the future were also discussed.
KW - Acrylic acid biosynthesis
KW - Enoyl-CoA hydratase
KW - Inexpensive carbon source
KW - Recombinant Escherichia coli
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U2 - 10.1007/s00253-015-7272-z
DO - 10.1007/s00253-015-7272-z
M3 - Article
C2 - 26782744
AN - SCOPUS:84954537556
SN - 0175-7598
VL - 100
SP - 4901
EP - 4907
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 11
ER -