TY - JOUR
T1 - Biotin deficiency decreases life span and fertility but increases stress resistance in Drosophila melanogaster
AU - Landenberger, Anette
AU - Kabil, Hadise
AU - Harshman, Lawrence G.
AU - Zempleni, Janos
N1 - Funding Information:
We thank Yue Wang for her expert help in fertility experiments. This work was supported by National Institutes of Health grants DK 60447 and 1R24 GM65513, and by United States Army grant DAAD19-03-1-0152. This article is a contribution of the University of Nebraska Agricultural Research Division, Lincoln, NE 68583 (Journal Series No. 14374).
PY - 2004/10
Y1 - 2004/10
N2 - Biotin deficiency is associated with fetal malformations and activation of cell survival pathways in mammals. In this study we determined whether biotin status affects life span, stress resistance, and fertility in the fruit fly Drosophila melanogaster. Male and female flies of the Canton-S strain had free access to diets containing 6.0 (control), 4.8, 2.5, or 0 pmol biotin/100 mg. Biotin concentrations in diets correlated with activities of biotin-dependent propionyl-CoA carboxylase and biotin concentrations in fly homogenates, but not with biotinylation of histones (DNA-binding proteins). Propionyl-CoA carboxylase activities and biotin concentrations were lower in males than in females fed diets low in biotin. The life span of biotin-deficient males and females was up to 30% shorter compared to biotin-sufficient controls. Exposure to oxidative stress reversed the effects of biotin status on survival in male flies: survival times increased by 40% in biotin-deficient males compared to biotin-sufficient controls. Biotin status did not affect survival of females exposed to oxidative stress. Exposure of flies to cold, heat, and oxidative stress was associated with mobilization of biotin from yet unknown sources. Biotin deficiency decreased fertility of flies. When biotin-deficient males and females were mated, the hatching rate (larvae hatched per egg) decreased by about 28% compared to biotin-sufficient controls. These findings are consistent with the hypothesis that biotin affects life span, stress resistance, and fertility in fruit flies.
AB - Biotin deficiency is associated with fetal malformations and activation of cell survival pathways in mammals. In this study we determined whether biotin status affects life span, stress resistance, and fertility in the fruit fly Drosophila melanogaster. Male and female flies of the Canton-S strain had free access to diets containing 6.0 (control), 4.8, 2.5, or 0 pmol biotin/100 mg. Biotin concentrations in diets correlated with activities of biotin-dependent propionyl-CoA carboxylase and biotin concentrations in fly homogenates, but not with biotinylation of histones (DNA-binding proteins). Propionyl-CoA carboxylase activities and biotin concentrations were lower in males than in females fed diets low in biotin. The life span of biotin-deficient males and females was up to 30% shorter compared to biotin-sufficient controls. Exposure to oxidative stress reversed the effects of biotin status on survival in male flies: survival times increased by 40% in biotin-deficient males compared to biotin-sufficient controls. Biotin status did not affect survival of females exposed to oxidative stress. Exposure of flies to cold, heat, and oxidative stress was associated with mobilization of biotin from yet unknown sources. Biotin deficiency decreased fertility of flies. When biotin-deficient males and females were mated, the hatching rate (larvae hatched per egg) decreased by about 28% compared to biotin-sufficient controls. These findings are consistent with the hypothesis that biotin affects life span, stress resistance, and fertility in fruit flies.
KW - Biotin
KW - Drosophila melanogaster
KW - Fertility
KW - Life span
KW - Stress
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U2 - 10.1016/j.jnutbio.2004.04.006
DO - 10.1016/j.jnutbio.2004.04.006
M3 - Article
C2 - 15542350
AN - SCOPUS:7944234368
SN - 0955-2863
VL - 15
SP - 591
EP - 600
JO - Journal of Nutritional Biochemistry
JF - Journal of Nutritional Biochemistry
IS - 10
ER -