TY - JOUR
T1 - Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1
AU - Leng, Yifei
AU - Bao, Jianguo
AU - Chang, Gaofeng
AU - Zheng, Han
AU - Li, Xingxing
AU - Du, Jiangkun
AU - Snow, Daniel
AU - Li, Xu
N1 - Funding Information:
This study was supported by Natural Science Foundation of China (No. 41373083 ), and US National Science Foundation ( CBET-1351676 ).
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/11/15
Y1 - 2016/11/15
N2 - Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 °C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil.
AB - Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 °C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil.
KW - Biotransformation
KW - Hydrolysis
KW - Stenotrophomonas maltophilia
KW - Tetracycline
KW - Transformation products
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U2 - 10.1016/j.jhazmat.2016.06.053
DO - 10.1016/j.jhazmat.2016.06.053
M3 - Article
C2 - 27420384
AN - SCOPUS:84978180012
VL - 318
SP - 125
EP - 133
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
SN - 0304-3894
ER -