Blood monocytes attenuate lung fibroblast contraction of three-dimensional collagen gels in coculture

C. M. Sköld, X. D. Liu, T. Umino, Y. K. Zhu, R. F. Ertl, D. J. Romberger, S. I. Rennard

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Mononuclear phagocytes can interact with mesenchymal cells and extracellular matrix components that are crucial for connective tissue rearrangement. We asked whether blood monocytes can alter matrix remodeling mediated by human lung fibroblasts cultured in a three-dimensional collagen gel. Blood monocytes from healthy donors (>95% pure) were cast into type I collagen gels that contained lung fibroblasts. Monocytes in coculture inhibited the fibroblast-mediated gel contractility in a time- and concentration-dependent manner. The concentration of PGE2, a well-known inhibitor of gel contraction, was higher (P < 0.01) in media from coculture; this media attenuated fibroblast gel contraction, whereas conditioned media from either cell type cultured alone did not. Three-dimensional cultured monocytes responded to conditioned media from cocultures by producing interleukin-1β and tumor necrosis factor-α, whereas fibroblasts increased synthesis of PGE2. Antibodies to interleukin-1β and tumor necrosis factor-α blocked the monocyte inhibitory effect and reduced the amount of PGE2 produced. The ability of monocytes to block the fibroblast contraction of matrix may be an important mechanism in regulating tissue remodeling.

Original languageEnglish (US)
Pages (from-to)L667-L674
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number4 23-4
StatePublished - 2000


  • Interleukin-1β
  • Matrix
  • Prostaglandin E
  • Remodeling
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Fingerprint Dive into the research topics of 'Blood monocytes attenuate lung fibroblast contraction of three-dimensional collagen gels in coculture'. Together they form a unique fingerprint.

Cite this