Blood volume determination in the mouse

A. C. Riches; J. G. Sharp; D. Brynmor Thomas; S. Vaughan Smith

doi:10.1113/jphysiol.1973.sp010086

Blood volume determination in the mouse

A. C. Riches, J. G. Sharp, D. Brynmor Thomas, S. Vaughan Smith

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

1. The blood volume of the mouse has been measured using ⁵⁹Fe‐labelled red cells to determine the red cell volume and ¹³¹I‐labelled human serum albumin to determine the plasma volume. 2. Values for the blood volume of 95·0 ± 1·5, 96·3 ± 2·7 and 84·7 ± 1·2 ml./kg body wt. were found for CSI female, CBA female and CBA male mice respectively. 3. A marked discrepancy was observed between the venous (cardiac) haematocrit and the whole body haematocrit. 4. The blood volume of the mouse must be determined from the red cell volume and the plasma volume, measured using appropriate labels, and not from the red cell volume or the plasma volume using the venous haematocrit.

Original language	English (US)
Pages (from-to)	279-284
Number of pages	6
Journal	The Journal of Physiology
Volume	228
Issue number	2
DOIs	https://doi.org/10.1113/jphysiol.1973.sp010086
State	Published - Jan 1 1973
Externally published	Yes

ASJC Scopus subject areas

Physiology

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abstract = "1. The blood volume of the mouse has been measured using 59Fe‐labelled red cells to determine the red cell volume and 131I‐labelled human serum albumin to determine the plasma volume. 2. Values for the blood volume of 95·0 ± 1·5, 96·3 ± 2·7 and 84·7 ± 1·2 ml./kg body wt. were found for CSI female, CBA female and CBA male mice respectively. 3. A marked discrepancy was observed between the venous (cardiac) haematocrit and the whole body haematocrit. 4. The blood volume of the mouse must be determined from the red cell volume and the plasma volume, measured using appropriate labels, and not from the red cell volume or the plasma volume using the venous haematocrit.",

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AU - Thomas, D. Brynmor

AU - Smith, S. Vaughan

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AB - 1. The blood volume of the mouse has been measured using 59Fe‐labelled red cells to determine the red cell volume and 131I‐labelled human serum albumin to determine the plasma volume. 2. Values for the blood volume of 95·0 ± 1·5, 96·3 ± 2·7 and 84·7 ± 1·2 ml./kg body wt. were found for CSI female, CBA female and CBA male mice respectively. 3. A marked discrepancy was observed between the venous (cardiac) haematocrit and the whole body haematocrit. 4. The blood volume of the mouse must be determined from the red cell volume and the plasma volume, measured using appropriate labels, and not from the red cell volume or the plasma volume using the venous haematocrit.

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Blood volume determination in the mouse

Abstract

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