Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor

R. Glenn Hammonds, Ralph Schwall, Andrew Dudley, Lucy Berkemeier, Cora Lai, James Lee, Noreen Cunningham, A. H. Reddi, William I. Wood, Anthony J. Mason

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalMolecular Endocrinology
Volume5
Issue number1
DOIs
StatePublished - Jan 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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