Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor

R. Glenn Hammonds; Ralph Schwall; Andrew Dudley; Lucy Berkemeier; Cora Lai; James Lee; Noreen Cunningham; A. H. Reddi; William I. Wood; Anthony J. Mason

doi:10.1210/mend-5-1-149

Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor

R. Glenn Hammonds, Ralph Schwall, Andrew Dudley, Lucy Berkemeier, Cora Lai, James Lee, Noreen Cunningham, A. H. Reddi, William I. Wood, Anthony J. Mason

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.

Original language	English (US)
Pages (from-to)	149-155
Number of pages	7
Journal	Molecular Endocrinology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1210/mend-5-1-149
State	Published - Jan 1991
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Endocrinology

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abstract = "The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.",

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AU - Hammonds, R. Glenn

AU - Schwall, Ralph

AU - Dudley, Andrew

AU - Berkemeier, Lucy

AU - Lai, Cora

AU - Lee, James

AU - Cunningham, Noreen

AU - Reddi, A. H.

AU - Wood, William I.

AU - Mason, Anthony J.

PY - 1991/1

Y1 - 1991/1

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Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor

Abstract

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