Bone morphogenetic protein‐2b stimulation of growth and osteogenic phenotypes in rat osteoblast‐like cells: Comparison with TGF‐β1

Theresa L. Chen; Rebecca L. Bates; Andrew Dudley; Glenn R. Hammonds; Edward P. Amento

doi:10.1002/jbmr.5650061216

Bone morphogenetic protein‐2b stimulation of growth and osteogenic phenotypes in rat osteoblast‐like cells: Comparison with TGF‐β₁

Theresa L. Chen, Rebecca L. Bates, Andrew Dudley, Glenn R. Hammonds, Edward P. Amento

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

The biologic effects of recombinant human bone morphogenetic protein‐2b (BMP‐2b = BMP‐4) were studied and compared with transforming growth factor‐β₁ (TGF‐β₁) in fetal rat osteoblast‐like (ROB) cells. Similar to the effects of TGF‐β₁, BMP‐2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF‐β₁, which inhibited alkaline phosphatase activity, BMP‐2b enhanced enzyme activity eight‐to ninefold over the control level. The present study demonstrates direct actions of BMP‐2b on bone‐associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP‐2b in vivo.

Original language	English (US)
Pages (from-to)	1387-1393
Number of pages	7
Journal	Journal of Bone and Mineral Research
Volume	6
Issue number	12
DOIs	https://doi.org/10.1002/jbmr.5650061216
State	Published - Dec 1991
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Orthopedics and Sports Medicine

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title = "Bone morphogenetic protein‐2b stimulation of growth and osteogenic phenotypes in rat osteoblast‐like cells: Comparison with TGF‐β1",

abstract = "The biologic effects of recombinant human bone morphogenetic protein‐2b (BMP‐2b = BMP‐4) were studied and compared with transforming growth factor‐β1 (TGF‐β1) in fetal rat osteoblast‐like (ROB) cells. Similar to the effects of TGF‐β1, BMP‐2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF‐β1, which inhibited alkaline phosphatase activity, BMP‐2b enhanced enzyme activity eight‐to ninefold over the control level. The present study demonstrates direct actions of BMP‐2b on bone‐associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP‐2b in vivo.",

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T2 - Comparison with TGF‐β1

AU - Chen, Theresa L.

AU - Bates, Rebecca L.

AU - Dudley, Andrew

AU - Hammonds, Glenn R.

AU - Amento, Edward P.

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AB - The biologic effects of recombinant human bone morphogenetic protein‐2b (BMP‐2b = BMP‐4) were studied and compared with transforming growth factor‐β1 (TGF‐β1) in fetal rat osteoblast‐like (ROB) cells. Similar to the effects of TGF‐β1, BMP‐2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF‐β1, which inhibited alkaline phosphatase activity, BMP‐2b enhanced enzyme activity eight‐to ninefold over the control level. The present study demonstrates direct actions of BMP‐2b on bone‐associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP‐2b in vivo.

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Bone morphogenetic protein‐2b stimulation of growth and osteogenic phenotypes in rat osteoblast‐like cells: Comparison with TGF‐β₁

Abstract

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