Bovine lactoferrin mRNA: Sequence, analysis, and expression in the mammary gland

Richard E. Goodman, Floyd L. Schanbacher

Research output: Contribution to journalArticlepeer-review

87 Scopus citations

Abstract

The mRNA sequence for bovine lactoferrin expressed in the mammary gland was determined by sequencing three over lapping cDNA clones and by direct sequencing of the mRNA. The mRNA (2351 bases) codes for a 708 amino acid protein with a 19 amino acid signal peptide immediately preceding a sequence identical to the N-terminal 40 amino acids reported for bovine lactoferrin (1). A putative destabilizing sequence (AUUUA) was identified in the 3′-untranslated region. The nucleic acid sequence and deduced amino acid sequence are highly homologous with other transferrin family members. Lactoferrin mRNA concentrations in bovine mammary tissue were quite low two days before parturition and during lactation but were high three days after the cessation of milking, a sharp contrast from the pattern of regulation of the other milk proteins.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number1
DOIs
StatePublished - Oct 15 1991
Externally publishedYes

Keywords

  • aa
  • amino acid
  • bLf
  • bovine lactoferrin
  • hLf
  • hTf
  • human lactoferrin
  • human transferrin
  • mLf
  • mouse lactoferrin
  • oTf,
  • ovotransferrin
  • pTf
  • porcine transferrin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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