Bovine mammary alveolar MAC-T cells afford a tool for studies of bovine milk exosomes in drug delivery

Mojisola Ogunnaike, Haichuan Wang, Janos Zempleni

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine milk exosomes (BMEs) have attracted attention as vehicles for delivering RNA therapeutics. BMEs originate in mammary alveolar cells. Here, we determined whether bovine mammary alveolar MAC-T cells afford a tool to assess RNA delivery by BMEs. MAC-T cells exosomes (MAC-T BMEs) and BMEs were harvested by differential ultracentrifugation. Exosome size, morphology, microRNA content and marker proteins were assessed using nanoparticle tracking analysis, transmission electron microscopy, real-time PCR and immunoblot analysis, respectively. MAC-T cells were genetically engineered to secrete MAC-T BMEs endogenously labeled with a near-infrared fluorescent protein and tissue distribution was compared to fluorophore-labeled BMEs following intravenous injection in C57BL/6 mice. Morphology and size were similar in MAC-T BMEs and BMEs (94 ± 5.8 nm and 101 ± 4.2 nm, p > 0.05). Both preparations expressed miR-320a, miR-200c and let-7a-5p (positive controls) but not miR-1 (negative control). Exosome marker proteins, CD9, CD63, CD81 and Tsg101, were detected in both MAC-T BMEs and BMEs. Distribution in mouse tissues was similar for both preparations, with liver being the primary accumulation site. Collectively, MAC-T BMEs afford a tool for BMEs-based RNA delivery studies.

Original languageEnglish (US)
Article number121263
JournalInternational journal of pharmaceutics
Volume610
DOIs
StatePublished - Dec 15 2021

Keywords

  • Bovine mammary alveolar MAC-T cells
  • Bovine milk exosomes
  • CD81 fusion protein
  • Drug delivery
  • RNA
  • Transgenic

ASJC Scopus subject areas

  • Pharmaceutical Science

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