Brain ingress of regulatory T cells in a murine model of HIV-1 encephalitis

Nan Gong, Jianuo Liu, Ashley D. Reynolds, Santhi Gorantla, R. Lee Mosley, Howard E. Gendelman

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

CD4+CD25+ regulatory T cells (Treg) transform the HIV-1 infected macrophage from a neurotoxic to a neuroprotective phenotype. This was demonstrated previously in a murine model of HIV-1 encephalitis induced by intracranial injection of HIV-1/vesicular stomatitis virus-infected bone marrow macrophages. In this report, relationships between Treg ingress of end organ tissues, notably the brain, and neuroprotection were investigated. Treg from EGFP-transgenic donor mice were expanded, labeled with indium-111, and adoptively transferred. Treg distribution was assayed by single photon emission computed tomography and immunohistochemistry. Treg readily migrated across the blood brain barrier and were retained within virus-induced neuroinflammatory sites. In non-inflamed peripheral tissues (liver and spleen) Treg were depleted. These observations demonstrate that Treg migrate to sites of inflammation where they modulate immune responses.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalJournal of Neuroimmunology
Volume230
Issue number1-2
DOIs
StatePublished - Jan 2011

Keywords

  • Blood brain barrier
  • CD4+CD25+ regulatory T cells
  • HIV-1
  • Macrophage
  • Neuroprotection
  • Single photon emission computed tomography

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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