TY - JOUR
T1 - Brain region mapping using global metabolomics
AU - Ivanisevic, Julijana
AU - Epstein, Adrian A.
AU - Kurczy, Michael E.
AU - Benton, Paul H.
AU - Uritboonthai, Winnie
AU - Fox, Howard S.
AU - Boska, Michael D.
AU - Gendelman, Howard E.
AU - Siuzdak, Gary
N1 - Funding Information:
We gratefully acknowledge financial support from NIH grants P01 DA026146-02 (G.S.), P01 DA028555, R01 NS36126, P01 NS31492, 2R01 NS034239, P01 MH64570, P01 NS43985, R01 AG043540 (H.E.G.), and P30 MH062261 (G.S., H.S.F., M.D.B., and H.E.G.), and from the Nebraska Research Initiative (M.D.B.), and by the University of Nebraska Foundation, which includes individual donations from Dr. Carol Swarts and Frances and Louie Blumkin and the Vice Chancellor’s office of the University of Nebraska Medical Center (H.E.G.). The Center for Translational Mouse Models at the University of Nebraska Medical Center supplied the NSG immunodeficient mice for studies.
Publisher Copyright:
© 2014 Elsevier Ltd. All rights reserved.
PY - 2014/11/20
Y1 - 2014/11/20
N2 - Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.
AB - Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.
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U2 - 10.1016/j.chembiol.2014.09.016
DO - 10.1016/j.chembiol.2014.09.016
M3 - Article
C2 - 25457182
AN - SCOPUS:84911917414
SN - 2451-9448
VL - 21
SP - 1575
EP - 1584
JO - Cell Chemical Biology
JF - Cell Chemical Biology
IS - 11
ER -