Brain region mapping using global metabolomics

Julijana Ivanisevic, Adrian A. Epstein, Michael E. Kurczy, Paul H. Benton, Winnie Uritboonthai, Howard S. Fox, Michael D. Boska, Howard E. Gendelman, Gary Siuzdak

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.

Original languageEnglish (US)
Pages (from-to)1575-1584
Number of pages10
JournalChemistry and Biology
Volume21
Issue number11
DOIs
StatePublished - Nov 20 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Brain region mapping using global metabolomics'. Together they form a unique fingerprint.

  • Cite this

    Ivanisevic, J., Epstein, A. A., Kurczy, M. E., Benton, P. H., Uritboonthai, W., Fox, H. S., Boska, M. D., Gendelman, H. E., & Siuzdak, G. (2014). Brain region mapping using global metabolomics. Chemistry and Biology, 21(11), 1575-1584. https://doi.org/10.1016/j.chembiol.2014.09.016