Bronchoalveolar lavage exosomes in lipopolysaccharide-induced septic lung injury

Zhihong Yuan, Brahmchetna Bedi, Ruxana T. Sadikot

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) represent a heterogeneous group of lung diseases which continues to have a high morbidity and mortality. The molecular pathogenesis of ALI is being better defined; however, because of the complex nature of the disease molecular therapies have yet to be developed. Here we use a lipopolysaccharide (LPS) induced mouse model of acute septic lung injury to delineate the role of exosomes in the inflammatory response. Using this model, we were able to show that mice that are exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid from the lungs that are packaged with miRNA and cytokines which regulate inflammatory response. Further using a co-culture model system, we show that exosomes released from macrophages disrupt expression of tight junction proteins in bronchial epithelial cells. These results suggest that 1) cross talk between innate immune and structural cells through the exosomal shuttling contribute to the inflammatory response and disruption of the structural barrier and 2) targeting these miRNAs may provide a novel platform to treat ALI and ARDS.

Original languageEnglish (US)
Article numbere57737
JournalJournal of Visualized Experiments
Volume2018
Issue number135
DOIs
StatePublished - May 21 2018
Externally publishedYes

Keywords

  • Exosomes
  • Genetics
  • Issue 135
  • LPS
  • Lung
  • MiR-146a
  • MiR-155
  • MicroRNAs
  • Sepsis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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