c-Myc promoter activation in medulloblastoma

I. Mei Siu, Ahita Lal, Jill R. Blankenship, Naji Aldosari, Gregory J. Riggins

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

The c-myc oncogene is commonly activated in medulloblastoma. Genomic amplification is a well-documented cause of c-myc activation but does not account for all cases of c-myc activation. In this study, we sought other means by which c-myc is overexpressed in medulloblastoma. Twelve medulloblastoma or PNET cell lines were screened for c-myc genomic amplification, mRNA levels, and protein levels. Two medulloblastoma lines, D283 Med and D721 Med, were identified that expressed c-myc mRNA and protein at high levels without genomic amplification. The c-myc gene's regulatory sequences were normal in those cell lines. However, specific regions of the promoter, independent of the β-catenin binding sites, were responsible for activation as revealed by promoter assays and site-directed mutagenesis. Transcriptional activation by a β-catenin-independent pathway is therefore a likely mechanism for c-myc overexpression in a subset of medulloblastomas.

Original languageEnglish (US)
Pages (from-to)4773-4776
Number of pages4
JournalCancer Research
Volume63
Issue number16
StatePublished - Aug 15 2003

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Siu, I. M., Lal, A., Blankenship, J. R., Aldosari, N., & Riggins, G. J. (2003). c-Myc promoter activation in medulloblastoma. Cancer Research, 63(16), 4773-4776.