Calcium/calmodulin-dependent protein kinase II activity regulates the proliferative potential of growth plate chondrocytes

Yuwei Li, Molly J. Ahrens, Amy Wu, Jennifer Liu, Andrew T. Dudley

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


For tissues that develop throughout embryogenesis and into postnatal life, the generation of differentiated cells to promote tissue growth is at odds with the requirement to maintain the stem cell/progenitor cell population to preserve future growth potential. In the growth plate cartilage, this balance is achieved in part by establishing a proliferative phase that amplifies the number of progenitor cells prior to terminal differentiation into hypertrophic chondrocytes. Here, we show that endogenous calcium/calmodulin-dependent protein kinase II (CamkII, also known as Camk2) activity is upregulated prior to hypertrophy and that loss of CamkII function substantially blocks the transition from proliferation to hypertrophy. Wnt signaling and Pthrpinduced phosphatase activity negatively regulate CamkII activity. Release of this repression results in activation of multiple effector pathways, including Runx2- and β-catenin-dependent pathways. We present an integrated model for the regulation of proliferation potential by CamkII activity that has important implications for studies of growth control and adult progenitor/stem cell populations.

Original languageEnglish (US)
Pages (from-to)359-370
Number of pages12
Issue number2
StatePublished - Jan 15 2011
Externally publishedYes


  • Adult stem cells
  • CamkII
  • Cartilage
  • Chick
  • Chondrocyte maturation
  • Growth plate
  • Kinase
  • Mouse
  • Transit amplification

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


Dive into the research topics of 'Calcium/calmodulin-dependent protein kinase II activity regulates the proliferative potential of growth plate chondrocytes'. Together they form a unique fingerprint.

Cite this