TY - JOUR
T1 - Calorimetric and spectroscopic investigation of drug-DNA interactions
T2 - II. Dipyrandlam binding to poly d(AT)
AU - Marky, Luis A.
AU - Snyder, James G.
AU - Breslauer, Kenneth J.
N1 - Funding Information:
ACKNOWLEDGEMENTS This work was supported by the National Institutes of Health, GM23509, the Charles and Johanna Busch Memorial Fund, and the Research Corporation. The authors wish to thank Dr. Gerald Manning for helpful discussions.
PY - 1983/8/25
Y1 - 1983/8/25
N2 - We report the first calorimetric investigation of steroid diamine binding to a DNA duplex. Absorption spectroscopy, batch calorimetry, and differential scanning calorlmetry (DSC) have been used to detect, monitor, and therraodynamically characterize the binding of the steroid diamine, dipyrandium, to poly d(AT). The following theraodynamic data for the binding in 16 mM Na+ at 25°C have been obtained: δG° - -6.5 kcal/nol, δH° = +4.2 kcal/mol, and δS = +36 e.u. We interpret the endothermic binding enthalpy in terms of steroid-induced conformational changes in the duplex (e.g. "kinking"). The large positive entropy is interpreted in terms of binding-induced release of bound water and condensed sodium ions. The salt-dependence of the binding constant is interpreted in terms of dipyrandium site-binding involving only one of the two charged ends of the steroid. The optical and DSC curves for the unsaturated steroid-poly d(AT) complexes exhibit biphasic behavior. A comparison of the van't Hoff and the calorimetric transition enthalpies reveals that steroid binding reduces the cooperativlty of the transition.
AB - We report the first calorimetric investigation of steroid diamine binding to a DNA duplex. Absorption spectroscopy, batch calorimetry, and differential scanning calorlmetry (DSC) have been used to detect, monitor, and therraodynamically characterize the binding of the steroid diamine, dipyrandium, to poly d(AT). The following theraodynamic data for the binding in 16 mM Na+ at 25°C have been obtained: δG° - -6.5 kcal/nol, δH° = +4.2 kcal/mol, and δS = +36 e.u. We interpret the endothermic binding enthalpy in terms of steroid-induced conformational changes in the duplex (e.g. "kinking"). The large positive entropy is interpreted in terms of binding-induced release of bound water and condensed sodium ions. The salt-dependence of the binding constant is interpreted in terms of dipyrandium site-binding involving only one of the two charged ends of the steroid. The optical and DSC curves for the unsaturated steroid-poly d(AT) complexes exhibit biphasic behavior. A comparison of the van't Hoff and the calorimetric transition enthalpies reveals that steroid binding reduces the cooperativlty of the transition.
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U2 - 10.1093/nar/11.16.5701
DO - 10.1093/nar/11.16.5701
M3 - Article
C2 - 6889134
AN - SCOPUS:0021112495
SN - 0305-1048
VL - 11
SP - 5701
EP - 5715
JO - Nucleic acids research
JF - Nucleic acids research
IS - 16
ER -