TY - JOUR
T1 - Capturing native interactions
T2 - intrinsic methods to study chromatin conformation
AU - Rowley, M. Jordan
AU - Corces, Victor G.
N1 - Funding Information:
Work in the authors' laboratory was supported by U.S. Public Health Service Award R01 GM035463 (V.G.C.) and the Ruth L. Kirschstein National Research Service Award F32 GM113570 (M.J.R.) from the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2016 The Authors. Published under the terms of the CC BY 4.0 license
PY - 2016/12/1
Y1 - 2016/12/1
N2 - The 3D organization of chromatin controls gene expression through spatial interactions between genomic loci. FISH and 3C-based methods that are commonly used to study chromatin organization utilize chemical crosslinking, a step that may introduce biases in detectable chromatin interactions. In their recent study, Papantonis and colleagues (Brant et al,) developed alternative new methods of detecting chromatin contacts without the use of chemical crosslinking agents. These tools increase the resolution and confidence at which interactions can be identified, and may be informative for chromatin interaction dynamics.
AB - The 3D organization of chromatin controls gene expression through spatial interactions between genomic loci. FISH and 3C-based methods that are commonly used to study chromatin organization utilize chemical crosslinking, a step that may introduce biases in detectable chromatin interactions. In their recent study, Papantonis and colleagues (Brant et al,) developed alternative new methods of detecting chromatin contacts without the use of chemical crosslinking agents. These tools increase the resolution and confidence at which interactions can be identified, and may be informative for chromatin interaction dynamics.
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U2 - 10.15252/msb.20167438
DO - 10.15252/msb.20167438
M3 - Comment/debate
C2 - 27940491
AN - SCOPUS:85007609817
SN - 1744-4292
VL - 12
JO - Molecular Systems Biology
JF - Molecular Systems Biology
IS - 12
M1 - 897
ER -