Cardiac ankyrin repeat protein, a negative regulator of cardiac gene expression, is augmented in human heart failure

Oliver Zolk, Marcus Frohme, Alexander Maurer, Franz Werner Kluxen, Bernd Hentsch, Dimitri Zubakov, Jörg D. Hoheisel, Irving H. Zucker, Salvatore Pepe, Thomas Eschenhagen

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

The technique of representational difference analysis of cDNA has been applied to screen for differentially expressed genes in a canine model of pacing-induced heart failure. We identified the canine homolog of the cardiac ankyrin repeat protein (CARP) which has been shown to be involved in the regulation of the transcription of cardiac genes. To confirm the significance for human heart failure, cardiac tissue specimens obtained from non-failing donor hearts and from explanted hearts from patients with end-stage heart failure were investigated. CARP mRNA and protein levels were markedly increased in failing left ventricles. Interestingly, alterations in CARP expression were restricted to ventricular tissue and were not observed in atria. Fractionation experiments revealed that CARP was expressed predominantly in the nuclei consistent with the proposed function of CARP as a modulator of transcription. Together, these findings raise the possibility that augmented ventricular CARP expression may play a role in the pathogenesis of human heart failure.

Original languageEnglish (US)
Pages (from-to)1377-1382
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number5
DOIs
StatePublished - 2002

Keywords

  • CARP
  • Cardiac muscle
  • Dilated cardiomyopathy
  • Gene expression
  • Heart failure
  • Ischemic cardiomyopathy
  • Representational difference analysis of cDNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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