Carrier mediated transport of choline in rat lens

Howard M. Jernigan, Peter F. Kador, Jin H. Kinoshita

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Choline accumulation was studied in rat lenses incubated in TC-199 medium containing radiolabeled choline. Choline entered the lens and was rapidly phosphorylated. Phosphorylcholine did not readily escape the lens and continued to accumulate throughout 24 hr of incubation. Accumulation of choline displayed saturation kinetics and this saturability appeared to be a property of transport rather than a reflection of the properties of choline kinase. Countertransport of labeled choline from lenses preloaded with radiolabeled choline indicates that choline transport in rat lens is carrier mediated. The existence of a choline carrier would also be consistent with the kinetic data. Ethanolamine competed for the choline carrier, however a component of ethanolamine uptake was non-saturable at concentrations of ethanolamine or choline up to 5 mm. Choline and ethanolamine appeared to be phosphorylated by separate kinases in lens.

Original languageEnglish (US)
Pages (from-to)709-717
Number of pages9
JournalExperimental Eye Research
Issue number6
StatePublished - Jun 1981


  • carrier-mediated
  • choline
  • choline kinase
  • ethanolamine
  • ethanolamine kinase
  • lens
  • phosphorylation
  • phosphorylcholine
  • phosphorylethanolamine
  • transport

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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