Carthami Flos suppresses neutrophilic lung inflammation in mice, for which nuclear factor-erythroid 2-related factor-1 is required

Jeehye Kim, Juyoun Woo, Ji Hyo Lyu, Hyuk Hwan Song, Han Sol Jeong, Ki Tae Ha, Jun Yong Choi, Chang Woo Han, Kyung Seop Ahn, Sei Ryang Oh, Ruxana T. Sadikot, Kyun Ha Kim, Myungsoo Joo

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Carthami Flos (CF) is used in traditional Asian medicine to treat blood stagnation and its associated diseases in patients. While the underlying mechanism for this effect remains unknown, CF has been reported to activate Nrf2, a transcription factor that is critical in protecting from various inflammatory lung diseases including acute lung injury (ALI). Here, we examined whether CF has a therapeutic effect on lung inflammation and assessed the impact of Nrf2 on the effect of CF using an ALI mouse model. Treatment of bone marrow derived macrophages with standardized aqueous extract of CF (AECF) activated Nrf2, resulting in the expression of Nrf2 dependent genes including GCLC, NQO-1 and HO-1. While intranasal LPS treatment of wild type mice resulted in neutrophilic infiltration and a concomitant expression of pro-inflammatory cytokine genes in the lung, the hallmarks of ALI, an intratracheal spraying of AECF to the lung 2 h after LPS treatment suppressed the inflammatory response. By contrast, similar treatment in nrf2-/- mice with AECF failed to attenuate the inflammatory response. Thus, our results show that AECF attenuated neutrophilic lung inflammation in mice, which required Nrf2. Since AECF administration abrogates lung inflammation after LPS treatment, we propose CF as a potential therapeutics in the management of ALI.

Original languageEnglish (US)
Pages (from-to)470-478
Number of pages9
JournalPhytomedicine
Volume21
Issue number4
DOIs
StatePublished - Mar 15 2014
Externally publishedYes

Keywords

  • Carthami Flos
  • Lipopolysaccharide
  • Neutrophilic lung inflammation
  • Nuclear factor-erythroid 2-related factor-1
  • Therapeutic effect

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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