Cathepsin B2 measurement by sensitive fluorometric ammonia analysis

S. Taylor, V. Ninjoor, D. M. Dowd, A. L. Tappel

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

A procedure has been developed for the measurement of cathepsin B2 activity that is based upon highly sensitive fluorometric ammonia analysis. The fluorochrome results from the reaction of phthaldehyde and mercaptoethanol with ammonium salts at pH 7.4. The method is sensitive in the range of 5-400 nmoles ammonium salt/ml. The assay is highly specific for ammonia since amino acids, peptides, amines, and amides do not interfere. Likewise, other components of the cathepsin B2 reaction system do not interfere. The most distinct advantage of the method is that the awkward diffusion step of most routine ammonia analyses can be eliminated.

Original languageEnglish (US)
Pages (from-to)153-162
Number of pages10
JournalAnalytical Biochemistry
Volume60
Issue number1
DOIs
StatePublished - Jul 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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