Cathepsin B2 measurement by sensitive fluorometric ammonia analysis

S. Taylor; V. Ninjoor; D. M. Dowd; A. L. Tappel

doi:10.1016/0003-2697(74)90140-7

Cathepsin B2 measurement by sensitive fluorometric ammonia analysis

S. Taylor, V. Ninjoor, D. M. Dowd, A. L. Tappel

Research output: Contribution to journal › Article › peer-review

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Abstract

A procedure has been developed for the measurement of cathepsin B2 activity that is based upon highly sensitive fluorometric ammonia analysis. The fluorochrome results from the reaction of phthaldehyde and mercaptoethanol with ammonium salts at pH 7.4. The method is sensitive in the range of 5-400 nmoles ammonium salt/ml. The assay is highly specific for ammonia since amino acids, peptides, amines, and amides do not interfere. Likewise, other components of the cathepsin B2 reaction system do not interfere. The most distinct advantage of the method is that the awkward diffusion step of most routine ammonia analyses can be eliminated.

Original language	English (US)
Pages (from-to)	153-162
Number of pages	10
Journal	Analytical Biochemistry
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(74)90140-7
State	Published - Jul 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Cathepsin B2 measurement by sensitive fluorometric ammonia analysis",

abstract = "A procedure has been developed for the measurement of cathepsin B2 activity that is based upon highly sensitive fluorometric ammonia analysis. The fluorochrome results from the reaction of phthaldehyde and mercaptoethanol with ammonium salts at pH 7.4. The method is sensitive in the range of 5-400 nmoles ammonium salt/ml. The assay is highly specific for ammonia since amino acids, peptides, amines, and amides do not interfere. Likewise, other components of the cathepsin B2 reaction system do not interfere. The most distinct advantage of the method is that the awkward diffusion step of most routine ammonia analyses can be eliminated.",

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year = "1974",

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AU - Taylor, S.

AU - Ninjoor, V.

AU - Dowd, D. M.

AU - Tappel, A. L.

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N2 - A procedure has been developed for the measurement of cathepsin B2 activity that is based upon highly sensitive fluorometric ammonia analysis. The fluorochrome results from the reaction of phthaldehyde and mercaptoethanol with ammonium salts at pH 7.4. The method is sensitive in the range of 5-400 nmoles ammonium salt/ml. The assay is highly specific for ammonia since amino acids, peptides, amines, and amides do not interfere. Likewise, other components of the cathepsin B2 reaction system do not interfere. The most distinct advantage of the method is that the awkward diffusion step of most routine ammonia analyses can be eliminated.

AB - A procedure has been developed for the measurement of cathepsin B2 activity that is based upon highly sensitive fluorometric ammonia analysis. The fluorochrome results from the reaction of phthaldehyde and mercaptoethanol with ammonium salts at pH 7.4. The method is sensitive in the range of 5-400 nmoles ammonium salt/ml. The assay is highly specific for ammonia since amino acids, peptides, amines, and amides do not interfere. Likewise, other components of the cathepsin B2 reaction system do not interfere. The most distinct advantage of the method is that the awkward diffusion step of most routine ammonia analyses can be eliminated.

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Cathepsin B2 measurement by sensitive fluorometric ammonia analysis

Abstract

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