Abstract
Several cations were examined for their ability to specifically affect one of the 3 l-glutamate (l-Glu) binding sites in rat forebrain synaptic plasma membranes (i.e. Na+-dependent, Cl--dependent and Cl--independent). Na+-dependent binding was potently inhibited by K+ and NH4+ ions. Other monovalent cations testedd (Cs+, Li+, triethylammonium) had no effect on this binding site. Polyvalent cations (Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Cr3+) also had little effect on the Na+-dependent l-Glu binding site. Cl--dependent l-Glu binding was potently inhibited by Na+ ions but was not affected by other monovalent ions. All of the divalent cations were potent inhibitors of both Cl--dependent and -independent binding. The results show that these binding sites of l-Glu can be distinguished by their response to cations and suggest possible novel modes of regulation in vivo.
Original language | English (US) |
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Pages (from-to) | 319-322 |
Number of pages | 4 |
Journal | Brain Research |
Volume | 329 |
Issue number | 1-2 |
DOIs | |
State | Published - Mar 11 1985 |
Externally published | Yes |
Keywords
- acidic amino acid receptors
- ammonium ions
- l-glutamate
- sodium ions
- synaptic plasma membranes
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology