CD200: Association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma

Yuh Seog Jung; Paola D. Vermeer; Daniel W. Vermeer; Sang Jin Lee; Ah Ra Goh; Hyun Joo Ahn; John H. Lee

doi:10.1002/hed.23608

CD200: Association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma

Yuh Seog Jung, Paola D. Vermeer, Daniel W. Vermeer, Sang Jin Lee, Ah Ra Goh, Hyun Joo Ahn, John H. Lee

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Background. The purpose of this study was to characterize the expression of CD200, a membrane protein that functions in immune evasion, to examine its correlations with cancer stem cell (CSC)-like features and analyze its response to chemotherapy and radiation in human papillomavirus (HPV)-positive (+) and negative (-) head and neck squamous cell carcinomas (HNSCCs). Methods. CD200 expression was analyzed in several HNSCC cell lines. CD200 was overexpressed in HPV(+) murine tonsil epithelial cells, its effects on Shh and Bmi-1 were examined in vitro, and tumor growth and response to chemoradiation were analyzed in vitro and in vivo. Results. CD200 was diversely expressed and consistently associated with expression of Bmi-1 and Shh. Overexpression of CD200 induced Bmi-1 and Shh. Tumors grew similarly between C57BL/6 and Rag1^-/- C57BL/6 mice. CD200 expression enhanced the resistance to chemoradiation only in vivo. Conclusion. CD200 was related to CSC features and modulates response to chemoradiation in vivo. Attenuating this might be a potential therapeutic strategy.

Original language	English (US)
Pages (from-to)	327-335
Number of pages	9
Journal	Head and Neck
Volume	37
Issue number	3
DOIs	https://doi.org/10.1002/hed.23608
State	Published - Mar 1 2015
Externally published	Yes

Keywords

CD200
Human papillomavirus
Immune tolerance
Neoplastic stem cells
Oropharyngeal cancer

ASJC Scopus subject areas

Otorhinolaryngology

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10.1002/hed.23608

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title = "CD200: Association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma",

abstract = "Background. The purpose of this study was to characterize the expression of CD200, a membrane protein that functions in immune evasion, to examine its correlations with cancer stem cell (CSC)-like features and analyze its response to chemotherapy and radiation in human papillomavirus (HPV)-positive (+) and negative (-) head and neck squamous cell carcinomas (HNSCCs). Methods. CD200 expression was analyzed in several HNSCC cell lines. CD200 was overexpressed in HPV(+) murine tonsil epithelial cells, its effects on Shh and Bmi-1 were examined in vitro, and tumor growth and response to chemoradiation were analyzed in vitro and in vivo. Results. CD200 was diversely expressed and consistently associated with expression of Bmi-1 and Shh. Overexpression of CD200 induced Bmi-1 and Shh. Tumors grew similarly between C57BL/6 and Rag1-/- C57BL/6 mice. CD200 expression enhanced the resistance to chemoradiation only in vivo. Conclusion. CD200 was related to CSC features and modulates response to chemoradiation in vivo. Attenuating this might be a potential therapeutic strategy.",

keywords = "CD200, Human papillomavirus, Immune tolerance, Neoplastic stem cells, Oropharyngeal cancer",

author = "Jung, {Yuh Seog} and Vermeer, {Paola D.} and Vermeer, {Daniel W.} and Lee, {Sang Jin} and Goh, {Ah Ra} and Ahn, {Hyun Joo} and Lee, {John H.}",

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year = "2015",

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doi = "10.1002/hed.23608",

language = "English (US)",

volume = "37",

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T2 - Association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma

AU - Jung, Yuh Seog

AU - Vermeer, Paola D.

AU - Vermeer, Daniel W.

AU - Lee, Sang Jin

AU - Goh, Ah Ra

AU - Ahn, Hyun Joo

AU - Lee, John H.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Background. The purpose of this study was to characterize the expression of CD200, a membrane protein that functions in immune evasion, to examine its correlations with cancer stem cell (CSC)-like features and analyze its response to chemotherapy and radiation in human papillomavirus (HPV)-positive (+) and negative (-) head and neck squamous cell carcinomas (HNSCCs). Methods. CD200 expression was analyzed in several HNSCC cell lines. CD200 was overexpressed in HPV(+) murine tonsil epithelial cells, its effects on Shh and Bmi-1 were examined in vitro, and tumor growth and response to chemoradiation were analyzed in vitro and in vivo. Results. CD200 was diversely expressed and consistently associated with expression of Bmi-1 and Shh. Overexpression of CD200 induced Bmi-1 and Shh. Tumors grew similarly between C57BL/6 and Rag1-/- C57BL/6 mice. CD200 expression enhanced the resistance to chemoradiation only in vivo. Conclusion. CD200 was related to CSC features and modulates response to chemoradiation in vivo. Attenuating this might be a potential therapeutic strategy.

AB - Background. The purpose of this study was to characterize the expression of CD200, a membrane protein that functions in immune evasion, to examine its correlations with cancer stem cell (CSC)-like features and analyze its response to chemotherapy and radiation in human papillomavirus (HPV)-positive (+) and negative (-) head and neck squamous cell carcinomas (HNSCCs). Methods. CD200 expression was analyzed in several HNSCC cell lines. CD200 was overexpressed in HPV(+) murine tonsil epithelial cells, its effects on Shh and Bmi-1 were examined in vitro, and tumor growth and response to chemoradiation were analyzed in vitro and in vivo. Results. CD200 was diversely expressed and consistently associated with expression of Bmi-1 and Shh. Overexpression of CD200 induced Bmi-1 and Shh. Tumors grew similarly between C57BL/6 and Rag1-/- C57BL/6 mice. CD200 expression enhanced the resistance to chemoradiation only in vivo. Conclusion. CD200 was related to CSC features and modulates response to chemoradiation in vivo. Attenuating this might be a potential therapeutic strategy.

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KW - Immune tolerance

KW - Neoplastic stem cells

KW - Oropharyngeal cancer

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CD200: Association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma

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