TY - JOUR
T1 - CD32 is expressed on cells with transcriptionally active HIV but does not enrich for HIV DNA in resting T cells
AU - Abdel-Mohsen, Mohamed
AU - Kuri-Cervantes, Leticia
AU - Grau-Exposito, Judith
AU - Spivak, Adam M.
AU - Nell, Racheal A.
AU - Tomescu, Costin
AU - Vadrevu, Surya Kumari
AU - Giron, Leila B.
AU - Serra-Peinado, Carla
AU - Genescà, Meritxell
AU - Castellví, Josep
AU - Wu, Guoxin
AU - Del Rio Estrada, Perla M.
AU - González-Navarro, Mauricio
AU - Lynn, Kenneth
AU - King, Colin T.
AU - Vemula, Sai
AU - Cox, Kara
AU - Wan, Yanmin
AU - Li, Qingsheng
AU - Mounzer, Karam
AU - Kostman, Jay
AU - Frank, Ian
AU - Paiardini, Mirko
AU - Hazuda, Daria
AU - Reyes-Terán, Gustavo
AU - Richman, Douglas
AU - Howell, Bonnie
AU - Tebas, Pablo
AU - Martinez-Picado, Javier
AU - Planelles, Vicente
AU - Buzon, Maria J.
AU - Betts, Michael R.
AU - Montaner, Luis J.
N1 - Funding Information:
We thank the HIV-1 patients who participated in the study and their providers; Y. A. Luna, M. F. Torres, S. P. Astorga Melendez, M. Becerril, V. Falcó, R. Willekens, and J. Navarro for referral of patients and sample collection; M. Buggert for critical evaluation of flow cytometric data; J. G. Prado for providing the viral stock R5-Bal; L. Luque and M. Fernández for technical assistance; and M. Ostrowski at the University of Toronto for providing HIV-infected PBMC samples. This work was supported by NIH R01 AI065279, U01 AI065279, and UM1 AI126620 (to L.J.M.); NIH R21 AI129636 and R21 NS106970 (to M.A.-M.); W. W. Smith Charitable Trust grant no. A17101 (to M.A.-M.); the Penn Center for AIDS Research (CFAR) (P30 AI 045008 to M.A.-M.); R21AI118411 (to M.J.B.); NIH R01 AI124843 (to V.P.); the Spanish Secretariat of Science and Innovation and FEDER funds (grants SAF2015-67334-R to M.J.B. and SAF2016-80033-R to J.M.-P.); and GeSIDA and the Spanish AIDS network Red Temática Cooperativa de nvestigación en SIDA (RD16/0025/0007 to M.J.B. and J.M.-P.). Additional support was provided by the Miguel Servet program funded by the Spanish Health Institute Carlos III (CP17/00179), the Spanish “Ministerio de Economía y Competitividad, Instituto de Salud Carlos III” (ISCIII, PI17/01470), the “Pla estratègic de recerca i innovació en salut” (PERIS), from the Catalan government for M.G., Collaboratory of AIDS Researchers for Eradication (CARE; UM1AI126619), BEAT-HIV (1UM1Al126620), the University of California, San Diego CFAR (AI306214), the Department of Veterans Affairs, and the James B. Pendleton Charitable Trust. Additional support was provided by The Philadelphia Foundation (Robert I. Jacobs Fund), Kean Family Professorship, Henry S. Miller Jr. and J. Kenneth Nimblett, AIDS funds from the Commonwealth of Pennsylvania and the Commonwealth Universal Research Enhancement Program, Pennsylvania Department of Health, the Penn CFAR (P30 AI 045008), and Cancer Center Grant (P30 CA10815).
Publisher Copyright:
Copyright © 2018 The Authors,
PY - 2018/4/18
Y1 - 2018/4/18
N2 - The persistence of HIV reservoirs, including latently infected, resting CD4+ T cells, is the major obstacle to cure HIV infection. CD32a expression was recently reported to mark CD4+ T cells harboring a replication-competent HIV reservoir during antiretroviral therapy (ART) suppression. We aimed to determine whether CD32 expression marks HIV latently or transcriptionally active infected CD4+ T cells. Using peripheral blood and lymphoid tissue of ART-treated HIV+ or SIV+ subjects, we found that most of the circulating memory CD32+ CD4+ T cells expressed markers of activation, including CD69, HLA-DR, CD25, CD38, and Ki67, and bore a TH2 phenotype as defined by CXCR3, CCR4, and CCR6. CD32 expression did not selectively enrich for HIV- or SIV-infected CD4+ T cells in peripheral blood or lymphoid tissue; isolated CD32+ resting CD4+ T cells accounted for less than 3% of the total HIV DNA in CD4+ T cells. Cell-associated HIV DNA and RNA loads in CD4+ T cells positively correlated with the frequency of CD32+ CD69+ CD4+ T cells but not with CD32 expression on resting CD4+ T cells. Using RNA fluorescence in situ hybridization, CD32 coexpression with HIV RNA or p24 was detected after in vitro HIV infection (peripheral blood mononuclear cell and tissue) and in vivo within lymph node tissue from HIV-infected individuals. Together, these results indicate that CD32 is not a marker of resting CD4+ T cells or of enriched HIV DNA–positive cells after ART; rather, CD32 is predominately expressed on a subset of activated CD4+ T cells enriched for transcriptionally active HIV after long-term ART.
AB - The persistence of HIV reservoirs, including latently infected, resting CD4+ T cells, is the major obstacle to cure HIV infection. CD32a expression was recently reported to mark CD4+ T cells harboring a replication-competent HIV reservoir during antiretroviral therapy (ART) suppression. We aimed to determine whether CD32 expression marks HIV latently or transcriptionally active infected CD4+ T cells. Using peripheral blood and lymphoid tissue of ART-treated HIV+ or SIV+ subjects, we found that most of the circulating memory CD32+ CD4+ T cells expressed markers of activation, including CD69, HLA-DR, CD25, CD38, and Ki67, and bore a TH2 phenotype as defined by CXCR3, CCR4, and CCR6. CD32 expression did not selectively enrich for HIV- or SIV-infected CD4+ T cells in peripheral blood or lymphoid tissue; isolated CD32+ resting CD4+ T cells accounted for less than 3% of the total HIV DNA in CD4+ T cells. Cell-associated HIV DNA and RNA loads in CD4+ T cells positively correlated with the frequency of CD32+ CD69+ CD4+ T cells but not with CD32 expression on resting CD4+ T cells. Using RNA fluorescence in situ hybridization, CD32 coexpression with HIV RNA or p24 was detected after in vitro HIV infection (peripheral blood mononuclear cell and tissue) and in vivo within lymph node tissue from HIV-infected individuals. Together, these results indicate that CD32 is not a marker of resting CD4+ T cells or of enriched HIV DNA–positive cells after ART; rather, CD32 is predominately expressed on a subset of activated CD4+ T cells enriched for transcriptionally active HIV after long-term ART.
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U2 - 10.1126/scitranslmed.aar6759
DO - 10.1126/scitranslmed.aar6759
M3 - Article
C2 - 29669853
AN - SCOPUS:85045856078
SN - 1946-6234
VL - 10
JO - Science translational medicine
JF - Science translational medicine
IS - 437
M1 - Y
ER -