CD43 is a murine T cell costimulatory receptor that functions independently of CD28

Anne I. Sperling, Jonathan M. Green, R. Lee Mosley, Peter L. Smith, Richard J. DiPaolo, John R. Klein, Jeffrey A. Bluestone, Craig B. Thompson

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Costimulation mediated by the CD28 receptor has been shown to play an important role in the development of a vigorous T cell immune response. Nevertheless, CD28-deficient mice can mount effective T cell-dependent immune responses. These data suggest that other costimulatory molecules may play a role in T cell activation. In a search for other costimulatory receptors on T ceils, we have characterized a monoclonal antibody (mAb) that can costimulate T cells in the absence of accessory cells. Similar to CD28 antibodies, this mAb, R2/60, was found to synergize with T cell receptor engagement in inducing proliferation. Independent ligation of CD3 and the ligand recognized by R2/60 results in T cell proliferation, suggesting that the two molecules do not have to colocalize to activate the R2/60 costimulatory pathway. R2/60 does not react with CD28, and furthermore, R2/60 costimulates in a CD28-independent fashion since the mAb costimulates T cells from the CD28-deficient mice as well as wild-type mice. Expression doning of the R2/60 antigen identified the ligand as murine CD43. Together, these data demonstrate that CD43 can serve as a receptor on T cells that can provide CD28-independent costimulation.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalJournal of Experimental Medicine
Volume182
Issue number1
DOIs
StatePublished - Jul 1 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Sperling, A. I., Green, J. M., Lee Mosley, R., Smith, P. L., DiPaolo, R. J., Klein, J. R., Bluestone, J. A., & Thompson, C. B. (1995). CD43 is a murine T cell costimulatory receptor that functions independently of CD28. Journal of Experimental Medicine, 182(1), 139-146. https://doi.org/10.1084/jem.182.1.139